Enhancement of selectivity and resolution in the enantioseparation of uncharged compounds using mixtures of oppositely charged cyclodextrins in capillary electrophoresis.
Abushoffa, Adel M; Fillet, Marianne; Servais, Anne-Catherineet al.
[en] The enantiomeric separation of some nonsteroidal anti-inflammatory drugs (NSAIDs) was investigated in capillary electrophoresis (CE) using dual systems with mixtures of charged cyclodextrin (CD) derivatives. A significant enhancement of selectivity and resolution could be achieved in the enantioseparation of these analytes in their uncharged form by the simultaneous addition of two oppositely charged CD derivatives to the background electrolyte. The combination of the single-isomer cationic CD, permethyl-6-monoamino-6-monodeoxy-beta-CD (PMMAbetaCD) and the single-isomer polyanionic CD, heptakis-6-sulfato-beta-cyclodextrin (HSbetaCD) in a pH 2.5 phosphoric acid-triethanolamine buffer, was designed and employed for the enantioseparation of profens. The improvement in selectivity and resolution can be attributed to the fact that the two CDs, which lead to independent and enantioselective complexation with the analyte enantiomers, have not only opposite effects on the electrophoretic mobility of these compounds but also opposite affinity patterns towards the enantiomers of these compounds. Binding constants for these enantiomers with each CD were determined using linear regression approach, in order to be able to predict the effect of the concentrations of the two CDs on enantiomeric selectivity and resolution in such dual systems.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Abushoffa, Adel M
Fillet, Marianne ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Servais, Anne-Catherine ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Hubert, Philippe ; Université de Liège - ULiège > Département de pharmacie > Chimie analytique
Crommen, Jacques ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Language :
English
Title :
Enhancement of selectivity and resolution in the enantioseparation of uncharged compounds using mixtures of oppositely charged cyclodextrins in capillary electrophoresis.
Publication date :
2003
Journal title :
Electrophoresis
ISSN :
0173-0835
eISSN :
1522-2683
Publisher :
Vch Publishers, Weinheim, Germany
Volume :
24
Issue :
3
Pages :
343-50
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]