Reference : CE-MS method development for peptides analysis, especially hepcidin, an iron metaboli...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/17025
CE-MS method development for peptides analysis, especially hepcidin, an iron metabolism marker.
English
Martin, Gaëlle mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Mansion, François mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Servais, Anne-Catherine mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Debrus, Benjamin mailto [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
Rozet, Eric mailto [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
Hubert, Philippe mailto [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
Crommen, Jacques mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Fillet, Marianne mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
2009
Electrophoresis
Vch Publishers
30
15
2624-31
Yes (verified by ORBi)
International
0173-0835
Weinheim
Germany
[en] Acetates/chemistry ; Acetonitriles/chemistry ; Animals ; Antimicrobial Cationic Peptides/analysis ; Cattle ; Electrophoresis, Capillary/methods ; Horses ; Humans ; Hydrogen-Ion Concentration ; Multivariate Analysis ; Osmolar Concentration ; Peptide Fragments/analysis ; Proteins/analysis ; Spectrometry, Mass, Electrospray Ionization/methods ; Temperature
[en] A method for the resolution of a peptides mixture including hepcidin-25, an iron metabolism marker, was developed by CE-ESI-MS. Several strategies were tested to optimize peptide separation, such as the addition of cyclodextrins or organic solvents in the BGE or the use of coated capillaries. Best results in terms of resolution, symmetry and efficiency were obtained with a BGE made of 500 mM ammonium acetate pH 4.5/ACN 70:30 v/v. Using the methodology of experimental design, BGE concentration, sheath liquid composition and MS-coupling parameters were then optimized in order to obtain the best signal intensity for hepcidin. Finally, a 225 mM BGE and a sheath liquid composed of isopropanol/water 80:20 v/v containing 0.5% v/v formic acid were selected as it constitutes the best compromise for selectivity, peak shape and sensitivity.
CIRM
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/17025
also: http://hdl.handle.net/2268/22565
10.1002/elps.200800794

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