Article (Scientific journals)
Inter-method variability in bone alkaline phosphatase measurement : clinical impact on the management of dialysis patients
CAVALIER, Etienne; Souberbielle, Jean-Claude; GADISSEUR, Romy et al.
2014In Clinical Biochemistry, 47 (13-14), p. 1227-30
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Keywords :
analytical variation; bone specific alkaline phosphatase; dialysis patients; standardization
Abstract :
[en] BACKGROUND: Bone-specific alkaline phosphatase (BAP) is now recommended to assess bone turnover in hemodialysis (HD) patients. However, little is known about potential variability between methods available to measure BAP. METHODS: We measured BAP in 76 HD patients with six different assays (Beckman-Coulter Ostase IRMA, Beckman-Coulter Ostase Access, IDS iSYS Ostase, IDS Ostase enzyme immunoassay, DiaSorin Liaison Ostase and Quidel MicroVue BAP). RESULTS: We observed a high correlation between all the assays ranging from 0.9948 (IDS iSYS vs. IDS EIA) to 0.9215 (DiaSorin Liaison vs. Quidel MicroVue). However, using the regression equations, the equivalent concentration of a Beckman-Coulter Access value of 10μg/L can range from 7.7 to 14.4μg/L and of 20μg/L can range from 16.9 to 27.9μg/L with other assays. According to Beckman-Coulter Access, 13%, 50% and 37% of the patients presented BAP values ≤10, between 10 and 20 and ≥20μg/L, respectively. Discrepancies are observed when other assays are used (concordance from 10 to 100%). CONCLUSIONS: Analytical problems leading to inter-method variation should be overcome to improve the usefulness of this marker in clinical practice. According to correlation results, recalibration of BAP assays is necessary but should not be a major issue.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
CAVALIER, Etienne  ;  Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
Souberbielle, Jean-Claude
GADISSEUR, Romy ;  Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
DUBOIS, Bernard ;  Centre Hospitalier Universitaire de Liège - CHU > Néphrologie
KRZESINSKI, Jean-Marie ;  Centre Hospitalier Universitaire de Liège - CHU > Néphrologie
DELANAYE, Pierre  ;  Centre Hospitalier Universitaire de Liège - CHU > Néphrologie
Language :
English
Title :
Inter-method variability in bone alkaline phosphatase measurement : clinical impact on the management of dialysis patients
Publication date :
September 2014
Journal title :
Clinical Biochemistry
ISSN :
0009-9120
eISSN :
1873-2933
Publisher :
Elsevier Science, Tarrytown, United States - New York
Volume :
47
Issue :
13-14
Pages :
1227-30
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 07 July 2014

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