Article (Scientific journals)
Training Modifies Innate Immune Responses in Blood Monocytes and in Pulmonary Alveolar Macrophages
Frellstedt, Linda; Waldschmidt, Ingrid; Gosset, Philippe et al.
2014In American Journal of Respiratory Cell and Molecular Biology, 51 (1), p. 135-142
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Keywords :
Exercise; innate immunity; monocytes; pulmonary alveolar macrophages; cytokine
Abstract :
[en] In humans, strenuous exercise causes increased susceptibility to respiratory infections associated with down-regulated expression of Toll-like receptors (TLRs), co-stimulatory and antigen-presenting molecules. Lower airway diseases are also a common problem in sport and racing horses. Because the innate immunity plays an essential role in lung defense mechanisms, we aimed to assess the effect of acute exercise and training on innate immune responses in two different compartments. Blood monocytes and pulmonary alveolar macrophages (PAM) were collected from horses in an untrained, moderately and intensively trained as well as deconditioned state before and after a strenuous exercise test (SET). The cells were analysed for TLR mRNA expression by real-time PCR in vitro and the cytokine production after in vitro stimulation with TLR ligands was measured by ELISA. Our results showed that training, but not acute exercise, modified the innate immune responses in both compartments. The mRNA expression of TLR3 was down-regulated by training in both cell types, whereas the expression of TLR4 was up-regulated in monocytes. Monocytes treated with lipopolysaccharide (LPS) and a synthetic diacylated lipoprotein (FSL) showed increased cytokine secretion in trained and deconditioned subjects indicating the activation of cells at the systemic level. The production of TNF-alpha and IFN-beta in non-stimulated and stimulated PAM was decreased in trained and deconditioned horses and might therefore explain the increased susceptibility to respiratory infections. Our study reports a dissociation between the systemic and the lung response to training that is probably implicated in the systemic inflammation and in the pulmonary susceptibility to infection.
Research center :
FARAH - Fundamental and Applied Research for Animals and Health - ULiège
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Veterinary medicine & animal health
Author, co-author :
Frellstedt, Linda ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Phys. neuro-muscul., de l'effort - Méd. sport. des animaux
Waldschmidt, Ingrid;  National Veterinary School Alfort, CIRALE, Goustranville, France
Gosset, Philippe;  Institut de Pasteur de Lille, CIIL, Lille, France
Desmet, Christophe  ;  Université de Liège - ULiège > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire
Pirottin, Dimitri ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Département de sciences fonctionnelles
Bureau, Fabrice ;  Université de Liège - ULiège > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire
Farnir, Frédéric  ;  Université de Liège - ULiège > Département de productions animales > Biostatistiques et bioinformatique appliquées aux sc. vétér.
Franck, Thierry  ;  Université de Liège - ULiège > Centre for Oxygen, Research & Development (CORD)
Dupuis-Tricaud, Marie-Capucine;  National Veterinary School Alfort, CIRALE, Goustranville, France
Lekeux, Pierre ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie
Art, Tatiana ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Phys. neuro-muscul., de l'effort - Méd. sport. des animaux
Language :
English
Title :
Training Modifies Innate Immune Responses in Blood Monocytes and in Pulmonary Alveolar Macrophages
Publication date :
2014
Journal title :
American Journal of Respiratory Cell and Molecular Biology
ISSN :
1044-1549
eISSN :
1535-4989
Publisher :
American Thoracic Society, New York, United States - New York
Volume :
51
Issue :
1
Pages :
135-142
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 July 2014

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