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A comparative study of embryogenic and non-embryogenic cell cultures in Picea abies (L.) Karst.
Du Jardin, Patrick; Chandelier, A.; Chauveau, F. et al.
1996In Somatic Cell Genetics and Molecular Genetics of Trees
 

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Keywords :
Somatic Embryogenesis; Zygotic Embryo; Mother Plant; Picea Abies
Abstract :
[en] Embryogenic cell cultures (or ESMs’,for embryonal-suspensor masses) and non embryogenic cultures (or ‘NEC’ for non embryogenic calli) have been initiated from young cotyledons in Picea abies (L.) Karst and separately multiplied for over two years. In order to better understand their physiological peculiarities, two molecular approaches were followed. First, bidimensional electrophoreses were used for comparing the polypeptide patterns in ESMs and NEC. Multiple extracellular polypeptides appeared specific to the embryogenic cell cultures, while several intracellular polypeptides were only observed in the non embryogenic calli, cultivated in the light or in darkness. Second, a mitochondrial DNA library was constructed in Picea abies and 168plasmid clones were used as RFLP probes for comparing the structure of the mitochondrial genome in the two types of cell material. Two probes detected extensive and qualitative DNA polymorphisms between embryogenic and non embryogenic cell cultures. Interestingly, the mtDNA pattern in ESMs was distinct from that of the corresponding mother plants (as defined in buds and needles), but apparent reversion to the mother plant pattern was observed upon embryo maturation. When comparing ESMs or NEC between genotypes, the two mtDNA probes detected extensive polymorphism, while the protein markers seemed to be genotype-independent.
Disciplines :
Genetics & genetic processes
Author, co-author :
Du Jardin, Patrick  ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Chandelier, A. ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Chauveau, F.
Bercetche, J.
Pâques, M.
Language :
English
Title :
A comparative study of embryogenic and non-embryogenic cell cultures in Picea abies (L.) Karst.
Publication date :
1996
Main work title :
Somatic Cell Genetics and Molecular Genetics of Trees
Publisher :
Elsevier
ISBN/EAN :
978-94-010-5761-5
Pages :
63-68
Commentary :
0-7923-4179-1
Available on ORBi :
since 17 June 2014

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