Article (Scientific journals)
Cell invasion in the spheroid sprouting assay: a spatial organisation analysis adaptable to cell behaviour.
Blacher, Silvia; Erpicum, Charlotte; Lenoir, Benedicte et al.
2014In PLoS ONE, 9 (5), p. 97019
Peer Reviewed verified by ORBi
 

Files


Full Text
Blacher S Plos 2014.pdf
Publisher postprint (3.16 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] The endothelial cell spheroid assay provides a suitable in vitro model to study (lymph) angiogenesis and test pro- and anti-(lymph) angiogenic factors or drugs. Usually, the extent of cell invasion, observed through optical microscopy, is measured. The present study proposes the spatial distribution of migrated cells as a new descriptor of the (lymph) angiogenic response. The utility of this novel method rests with its capacity to locally characterise spheroid structure, allowing not only the investigation of single and collective cell invasion but also the evolution of the spheroid core itself. Moreover, the proposed method can be applied to 2D-projected spheroid images obtained by optical microscopy, as well as to 3D images acquired by confocal microscopy. To validate the proposed methodology, endothelial cell invasion was evaluated under different experimental conditions. The results were compared with widely used global parameters. The comparison shows that our method prevents local spheroid modifications from being overlooked and leading to the possible misinterpretation of results.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Blacher, Silvia ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme
Erpicum, Charlotte ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Lenoir, Benedicte
Paupert, Jenny ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme
Moraes, Gustavo
Ormenese, Sandra ;  Université de Liège - ULiège > GIGA-Management : Pl-forme imagerie cell. et cytom. de flux
Bullinger, Eric ;  Université de Liège - ULiège > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Systèmes et modélisation : Méth. computat. pour la bio.syst.
Noël, Agnès ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme
Language :
English
Title :
Cell invasion in the spheroid sprouting assay: a spatial organisation analysis adaptable to cell behaviour.
Publication date :
2014
Journal title :
PLoS ONE
eISSN :
1932-6203
Publisher :
Public Library of Science, San Franscisco, United States - California
Volume :
9
Issue :
5
Pages :
e97019
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 14 June 2014

Statistics


Number of views
109 (10 by ULiège)
Number of downloads
302 (5 by ULiège)

Scopus citations®
 
46
Scopus citations®
without self-citations
40
OpenCitations
 
46

Bibliography


Similar publications



Contact ORBi