In vitro embryo production in goats: slaughterhouse and laparoscopic ovum pick up 2 (LOPU) derived oocytes have different kinetics and requirements regarding maturation 3 media
[en] A total of 3427 goat oocytes were used in this study in order to identify possible 24 differences during in vitro embryo production from slaughterhouse or laparoscopic ovum 25 pick up (LOPU) oocytes. In Experiment 1, one complex, one semi defined and one simplified 26 in vitro maturation (IVM) media were compared using slaughterhouse oocytes. In 27 Experiment 2, we checked the effect of oocyte origin (slaughterhouse or LOPU) on the 28 kinetics of maturation (18 vs. 22 vs. 26 h) when submitted to semi defined or simplified 29 media. In Experiment 3, we determined the differences in embryo development between 30 slaughterhouse and LOPU oocytes when submitted to both media and then to in vitro 31 fertilization (IVF) or parthenogenetic activation (PA). Embryos from all groups were vitrified 32 and their viability evaluated in vitro after thawing. In Experiment 1, no difference (P>0.05) 33 was detected among treatments for maturation rate (MII; 88% on average), cleavage (72%), 34 blastocyst from the initial number of cumulus oocyte complexes (COC; 46%) or from the 35 cleaved ones (63%), hatching rate (69%) and the total number of blastomeres (187). In 36 Experiment 2, there was no difference of MII rate between slaughterhouse oocytes cultured 37 for 18 or 22 h, whereas the MII rate increased significantly (P<0.05) between 18 and 22 h for 38 LOPU oocytes in the simplified medium. Moreover, slaughterhouse oocytes cultured in 39 simplified medium matured significantly faster than LOPU oocytes at 18 and 22 h (P<0.05). 40 In Experiment 3, cleavage rate was significantly greater (P<0.001) in all four groups of 41 embryos produced by PA than IVF. Interestingly, PA reached similar rates for slaughterhouse 42 oocytes cultured in both media, but improved (P<0.05) cleavage rate of LOPU oocytes. 43 Slaughterhouse oocytes had acceptable cleavage rate after IVF (~67%), whereas LOPU 44 oocytes displayed a lower one (~38%), in contrast to cleavage after PA. The percentage of 45 blastocysts in relation to cleaved embryos was not affected by the origin of the oocytes 46 (P>0.05). Therefore, slaughterhouse oocytes developed a greater proportion of blastocysts than LOPU ones, expressed as the percentage of total COC entering to IVM. Vitrified-thawed 48 blastocysts presented similar survival and hatching rates between the oocyte origin, media or 49 method of activation. In conclusion, slaughterhouse and LOPU derived oocytes may have 50 different IVM kinetics and require different IVM-IVF conditions. Although the IVM and IVF 51 systems still need improvements in order to enhance embryo yield, the in vitro development 52 (IVD) step is able to generate good quality embryos from LOPU derived oocytes.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Souza-Fabjan, JM; Institut Scientifique de Recherche Agronomique - INRA > UMR7247, Physiologie de la Reproduction et des Comportements, INRA, CNRS, 10 Université de Tours, Haras Nationaux. 37380 Nouzilly, France
Locatelli, Y; Institut Scientifique de Recherche Agronomique - INRA > UMR7247, Physiologie de la Reproduction et des Comportements, INRA, CNRS, 10 Université de Tours, Haras Nationaux. 37380 Nouzilly, France
Duffard, N; Institut Scientifique de Recherche Agronomique - INRA > UMR7247, Physiologie de la Reproduction et des Comportements, INRA, CNRS, 10 Université de Tours, Haras Nationaux. 37380 Nouzilly, France
Corbin, E; Institut Scientifique de Recherche Agronomique - INRA > UMR7247, Physiologie de la Reproduction et des Comportements, INRA, CNRS, 10 Université de Tours, Haras Nationaux. 37380 Nouzilly, France
Touzé, JL
Perreau, Ch
Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Freitas, VJ
Mermillod, P; Institut Scientifique de Recherche Agronomique - INRA > UMR7247, Physiologie de la Reproduction et des Comportements, INRA, CNRS, 10 Université de Tours, Haras Nationaux. 37380 Nouzilly, France
Language :
English
Title :
In vitro embryo production in goats: slaughterhouse and laparoscopic ovum pick up 2 (LOPU) derived oocytes have different kinetics and requirements regarding maturation 3 media
Cognié Y., Baril G., Poulin N., Mermillod P. Current status of embryo technologies in sheep and goat. Theriogenology 2003, 59:171-188.
Khatun M., Bhuiyan M.M., Ahmed J.U., Haque A., Rahman M.B., Shamsuddin M. Invitro maturation and fertilization of prepubertal and pubertal black Bengal goat oocytes. J Vet Sci 2011, 12:75-82.
Cognié Y., Poulin N., Locatelli Y., Mermillod P. State-of-the-art production, conservation and transfer of in-vitro-produced embryos in small ruminants. Reprod Fertil Dev 2004, 16:437-445.
Paramio M.T. Invivo and invitro embryo production in goats. Small Rumin Res 2010, 89:144-148.
Rodriguez-Dorta N., Cognié Y., Gonzalez F., Poulin N., Guignot F., Touzé J.L., et al. Effect of coculture with oviduct epithelial cells on viability after transfer of vitrified invitro produced goat embryos. Theriogenology 2007, 68:908-913.
Locatelli Y., Poulin N., Baril G., Touzé J.L., Fatet A., Beckers J.F., et al. Invitro maturation treatment affects developmental competence of laparoscopic ovum pickup-derived oocytes in follicle stimulating hormone-stimulated goats. Reprod Fertil Dev 2008, 20:182-183.
Rho G.J., Hahnel A., Betteridge K.J. Comparisons of oocyte maturation times and of three methods of sperm penetration for their effects on the production of goat embryos invitro. Theriogenology 2001, 56:503-516.
Taru Sharma G., Majumdar A.C., Bonde S.W. Chronology of maturational events in goat oocytes cultured invitro. Small Rumin Res 1996, 22:25-30.
Souza J.M.G., Duffard N., Bertoldo M.J., Locatelli Y., Corbin E., Fatet A., et al. Influence of heparin or the presence of cumulus cells during fertilization on the invitro production of goat embryos. Anim Reprod Sci 2013, 138:82-89.
Tan W.L., Wan Khadijah W.E., Abdullah R.B. Effect of sperm insemination duration on invitro fertilisation (IVF) performance on goats. Malas J Sci 2011, 30:105-111.
Mermillod P., Dalbies-Tran R., Uzbekova S., Thelie A., Traverso J.M., Perreau C., et al. Factors affecting oocyte quality: who is driving the follicle?. Reprod Domest Anim 2008, 43:393-400.
Traldi A.S., Leboeuf B., Cognié Y., Poulin N., Mermillod P. Comparative results of invitro and invivo survival of vitrified invitro produced goat and sheep embryos. Theriogenology 1999, 51:175.
Isachenko V., Alabart J.L., Dattena M., Nawroth F., Cappai P., Isachenko E., et al. New technology for vitrification and field (microscope-free) warming and transfer of small ruminant embryos. Theriogenology 2003, 59:1209-1218.
Guler A., Poulin N., Mermillod P., Terqui M., Cognié Y. Effect of growth factors, EGF and IGF-I, and estradiol on invitro maturation of sheep oocytes. Theriogenology 2000, 54:209-218.
Lonergan P., Rizos D., Kanka J., Nemcova L., Mbaye A.M., Kingston M., et al. Temporal sensitivity of bovine embryos to culture environment after fertilization and the implications for blastocyst quality. Reproduction 2003, 126:337-346.
Guignot F., Bouttier A., Baril G., Salvetti P., Pignon P., Beckers J.F., et al. Improved vitrification method allowing direct transfer of goat embryos. Theriogenology 2006, 66:1004-1011.
Dinnyes A., Carolan C., Lonergan P., Massip A., Mermillod P. Survival of frozen or vitrified bovine blastocysts produced invitro in synthetic oviduct fluid. Theriogenology 1996, 46:1425-1439.
Rodriguez-Gonzalez E., Lopez-Bejar M., Velilla E., Paramio M.T. Selection of prepubertal goat oocytes using the brilliant cresyl blue test. Theriogenology 2002, 57:1397-1409.
Shirazi A., Ardali M.A., Ahmadi E., Nazari H., Mamuee M., Heidari B. The effect of macromolecule source and type of media during invitro maturation of sheep oocytes on subsequent embryo development. J Reprod Infertil 2012, 13:13-19.
Korhonen K., Kananen K., Ketoja E., Matomäki J., Halmekytö M., Peippo J. Effects of serum-free invitro maturation of bovine oocytes on subsequent embryo development and cell allocation in two developmental stages of day 7 blastocysts. Reprod Domest Anim 2010, 45:42-49.
Koeman J., Keefer C.L., Baldassarre H., Downey B.R. Developmental competence of prepubertal and adult goat oocytes cultured in semi-defined media following laparoscopic recovery. Theriogenology 2003, 60:879-889.
Romaguera R., Casanovas A., Morató R., Izquierdo D., Catalá M., Jimenez-Macedo A.R., et al. Effect of follicle diameter on oocyte apoptosis, embryo development and chromosomal ploidy in prepubertal goats. Theriogenology 2010, 74:364-373.
Hammami S., Morato R., Romaguera R., Roura M., Catala M.G., Paramio M.T., et al. Developmental competence and embryo quality of small oocytes from pre-pubertal goats cultured in IVM medium supplemented with low level of hormones, insulin-transferrin-selenium and ascorbic acid. Reprod Domest Anim 2013, 48:339-344.
Graff K.J., Meintjes M., Han Y., Reggio B.C., Denniston R.S., Gavin W.G., et al. Comparing follicle stimulating hormone from two commercial sources for oocyte production from out-of-season dairy goats. J Dairy Sci 2000, 83:484-487.
Avelar S.R.G., Moura R.R., Sousa F.C., Pereira A.F., Almeida K.C., Melo C.H.S., et al. Oocyte production and invitro maturation in Canindé goats following hormonal ovarian stimulation. Anim Reprod 2012, 9:1-7.
Leoni G.G., Succu S., Satta V., Paolo M., Bogliolo L., Bebbere D., et al. Invitro production and cryotolerance of prepubertal and adult goat blastocysts obtained from oocytes collected by laparoscopic oocyte-pick-up (LOPU) after FSH treatment. Reprod Fertil Dev 2009, 21:901-908.
Paffoni A., Brevini T.A.L., Gandolfi F., Ragni G. Parthenogenetic activation: biology and applications in the ART laboratory. Placenta S 2008, 121-125.
Onger E.M., Bormann C.L., Bulter R.E., Melican D., Gavin W.G., Echlelard Y., et al. Development of goat embryos after invitro fertilization and parthenogenetic activation by different methods. Theriogenology 2001, 55:1933-1945.
Rizos D., Ward F., Duffy P., Boland M.P., Lonergan P. Consequences of bovine oocyte maturation, fertilization or early embryo development invitro versus invivo: implications for blastocyst yield and blastocyst quality. Mol Reprod Dev 2002, 61:234-248.
