Reference : Saccharomyces cerevisiae mitoproteome plasticity in response to recombinant alternati...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/1688
Saccharomyces cerevisiae mitoproteome plasticity in response to recombinant alternative ubiquinol oxidase
English
Mathy, Grégory [Université de Liège - ULg > Département des sciences de la vie > Bioénergétique et physiologie cellulaire >]
Navet, Rachel mailto [Université de Liège - ULg > > GIGA - Formation >]
Gerkens, Pascal [> > > >]
Leprince, Pierre mailto [Université de Liège - ULg > > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc. >]
De Pauw, Edwin mailto [Université de Liège - ULg > > Chimie physique, spectrométrie de masse >]
Sluse-Goffart, Claudine [> > > >]
Sluse, Francis mailto [Université de Liège - ULg > Département des sciences de la vie > Bioénergétique et physiologie cellulaire >]
Douette, Pierre [Université de Liège - ULg > > Bioénergétique et physiologie cellulaire >]
Feb-2006
Journal of Proteome Research
Amer Chemical Soc
5
2
339-348
Yes (verified by ORBi)
International
1535-3893
Washington
[en] alternative oxidase ; yeast ; mitoproteome ; proteomics ; two-dimensional differential in-gel electrophoresis
[en] The energy-dissipating alternative oxidase (AOX) from Hansenula anomala, was expressed in Saccharomyces cerevisiae. The recombinant AOX was functional. A comparative analysis by two-dimensional differential in-gel electrophoresis (2D-DIGE) of mitochondrial protein patterns found in wild-type and recombinant AOX strains was performed. 60 proteins exhibiting a significant difference in their abundance were identified. Interestingly, proteins implicated in major metabolic pathways such as Krebs cycle and amino acid biosynthesis were up-regulated. Surprisingly, an up-regulation of the respiratory-chain complex III was associated with a down-regulation of the ATP synthase complex.
Fonds de la Recherche Fondamentale Collective d’Initiative des Chercheurs - FRFC
Researchers ; Students
http://hdl.handle.net/2268/1688

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