Reference : Optimisation and validation of a generic method for the LC assay of six corticosteroids ...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/1684
Optimisation and validation of a generic method for the LC assay of six corticosteroids and salicylic acid in dermopharmaceutical forms
English
Marini Djang'Eing'A, Roland mailto [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
Pantella, A. [> > > >]
Bimazubute, Marcel [ > > ]
Chiap, Patrice mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Hubert, Philippe mailto [Université de Liège - ULg > > Chimie analytique >]
Crommen, Jacques mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Mar-2002
Chromatographia
Vieweg
55
5-6
263-269
Yes
International
0009-5893
Wiesbaden
[en] column liquid chromatography ; corticosteroids ; salicylic acid ; dermatological formulations ; method validation
[en] A generic method was developed for the liquid chromatographic (LC) assay of six corticosteroids and salicylic acid in different dermatological formulations containing methylparaben and propylparaben or sorbic acid as preservatives. An adequate separation was obtained using octadecylsilica as stationary phase and a mixture of acetonitrile (MeCN) and 25 mM phosphate buffer of pH 3.5 as mobile phase. Segmented gradient elution was applied from 30 to 45% of MeCN in 9.9 minutes, then from 45 to 96% in 19.0 minutes. UV detection was performed at 240 nm. The method was validated. Very good results were obtained with respect to linearity selectivity, precision and accuracy.
http://hdl.handle.net/2268/1684

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