[en] Experiments were conducted in a 0.25m diameter bubble column to investigate the effect of medium composition on oxygen transfer rate.
Aqueous solutions, the composition of which mimics a mammalian cell culture medium, are used. The effect on oxygen transfer rate of additives
used to protect cells against local hydrodynamic stresses induced by bubble coalescence and bursting is addressed, in the range of operating
conditions (aeration rates) met in animal cell cultures.
The mass transfer coefficient, the liquid viscosity and surface tension, and the bubble size distribution are measured as a function of liquid
composition and of gas superficial velocity, allowing to decouple the effects of the different additive on kL and on a.
Disciplines :
Chemical engineering
Author, co-author :
Toye, Dominique ; Université de Liège - ULiège > Département de chimie appliquée > Génie de la réaction et des réacteurs chimiques
Galifi, A.
Salmon, Thierry ; Université de Liège - ULiège > Département de chimie appliquée > Département de chimie appliquée
Marchot, Pierre ; Université de Liège - ULiège > Département de chimie appliquée > Génie chimique - Systèmes polyphasiques
Verdin, Emeline ; Université de Liège - ULiège > Département de chimie appliquée > Génie chimique - Opérations physiques unitaires
Crine, Michel ; Université de Liège - ULiège > Département de chimie appliquée > Génie chimique - Opérations physiques unitaires
Language :
English
Title :
Influence of medium composition on oxygen transfer rate in animal cell culture
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