Reference : Influence of medium composition on oxygen transfer rate in animal cell culture
Scientific journals : Article
Engineering, computing & technology : Chemical engineering
http://hdl.handle.net/2268/16722
Influence of medium composition on oxygen transfer rate in animal cell culture
English
Toye, Dominique mailto [Université de Liège - ULg > Département de chimie appliquée > Génie de la réaction et des réacteurs chimiques >]
Galifi, A. [> >]
Salmon, Thierry mailto [Université de Liège - ULg > Département de chimie appliquée > Département de chimie appliquée >]
Marchot, Pierre mailto [Université de Liège - ULg > Département de chimie appliquée > Génie chimique - Systèmes polyphasiques >]
Verdin, Emeline mailto [Université de Liège - ULg > Département de chimie appliquée > Génie chimique - Opérations physiques unitaires >]
Crine, Michel mailto [Université de Liège - ULg > Département de chimie appliquée > Génie chimique - Opérations physiques unitaires >]
2010
Canadian Journal of Chemical Engineering
Chemical Institute of Canada
88
4
671-676
Yes (verified by ORBi)
International
0008-4034
Ottawa
Canada
[en] kLa ; interfacial area ; bubble diameter ; optical probe ; disengagement technique
[en] Experiments were conducted in a 0.25m diameter bubble column to investigate the effect of medium composition on oxygen transfer rate.
Aqueous solutions, the composition of which mimics a mammalian cell culture medium, are used. The effect on oxygen transfer rate of additives
used to protect cells against local hydrodynamic stresses induced by bubble coalescence and bursting is addressed, in the range of operating
conditions (aeration rates) met in animal cell cultures.
The mass transfer coefficient, the liquid viscosity and surface tension, and the bubble size distribution are measured as a function of liquid
composition and of gas superficial velocity, allowing to decouple the effects of the different additive on kL and on a.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/16722

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