Article (Scientific journals)
Comprehensive plasma profiling for the characterization of graft-versus-host disease biomarkers
De Bock, Muriel; BEGUIN, Yves; Leprince, Pierre et al.
2014In Talanta, 125, p. 265-275
Peer Reviewed verified by ORBi
 

Files


Full Text
Comprehensive-plasma-profiling-for-the-characterization-of-graft-versus-host-disease-biomarkers_2014_Talanta.pdf
Publisher postprint (1.41 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Graft-versus-host disease; biomarkers; composite panel; clinical proteomics
Abstract :
[en] Acute graft-versus-host disease (aGVHD) remains a life-threatening complication of hematopoietic stem cell transplantation (HSCT), limiting its application. To optimize management of aGVHD and reduce therapy-related toxicity, early specific markers are needed. The main objective of this study was thus to uncover diagnostic biomarkers comparing plasma protein profiles of patients at the time of acute GVHD diagnosis and of patients undergoing HSCT without aGVHD. Additional analysis of samples taken 15 days before aGVHD diagnosis was also performed to evaluate the potential of the newly discovered biomarkers for early diagnosis. To extract a maximum of information from plasma samples, we used three complementary proteomic approaches, namely 2D-DIGE, SELDI-TOF-MS and 2D-LC-MSE. We identified and confirmed by means of a independent techniques, the differential expression of several proteins indicating significantly increased inflammation response and disturbance in the coagulation cascade. The variation of these proteins was already observed 15 days before GVHD diagnosis, suggesting the potential early detection of the disease before symptoms appearance. Finally, logistic regression analysis determines a composite biomarker panel comprising fibrinogen, fragment of fibrinogen beta chain, SAA, prothrombin fragments, apolipoprotein A1 and hepcidin that optimally discriminated patients with and without GVHD. The area under the receiver operating characteristic curve distinguishing these 2 groups was 0.95.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
De Bock, Muriel
BEGUIN, Yves  ;  Centre Hospitalier Universitaire de Liège - CHU > Hématologie clinique
Leprince, Pierre ;  Université de Liège - ULiège > GIGA - Neurosciences
WILLEMS, Evelyne ;  Centre Hospitalier Universitaire de Liège - CHU > Hématologie clinique
BARON, Frédéric  ;  Centre Hospitalier Universitaire de Liège - CHU > Hématologie clinique
Deroyer, Céline  ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Seidel, Laurence  ;  Université de Liège - ULiège > Département des sciences de la santé publique > Informatique médicale et biostatistique
CAVALIER, Etienne  ;  Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
DE SENY, Dominique ;  Centre Hospitalier Universitaire de Liège - CHU > Rhumatologie
MALAISE, Michel ;  Centre Hospitalier Universitaire de Liège - CHU > Rhumatologie
GOTHOT, André ;  Centre Hospitalier Universitaire de Liège - CHU > Hématologie biologique et immuno hématologie
MERVILLE, Marie-Paule ;  Centre Hospitalier Universitaire de Liège - CHU > Laboratoire NDB
Fillet, Marianne ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
More authors (3 more) Less
Language :
English
Title :
Comprehensive plasma profiling for the characterization of graft-versus-host disease biomarkers
Publication date :
July 2014
Journal title :
Talanta
ISSN :
0039-9140
eISSN :
1873-3573
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
125
Pages :
265-275
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 16 April 2014

Statistics


Number of views
131 (39 by ULiège)
Number of downloads
8 (8 by ULiège)

Scopus citations®
 
9
Scopus citations®
without self-citations
9
OpenCitations
 
9

Bibliography


Similar publications



Contact ORBi