Article (Scientific journals)
Strategies for Using the Sheep Ovarian Cortex as a Model in Reproductive Medicine
Fransolet, Maïté; Labied, Soraya; Henry, Laurie et al.
2014In PLoS ONE, 9 (3), p. 91073
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Abstract :
[en] Objective: To evaluate and compare the distribution and density of primordial follicles within a whole sheep ovary and to gain insight into how to overcome the impact of natural follicular heterogeneity on the experimental results. Design: Histological study. Setting: Academic research center. Animals: Five- to nine-month-old ewes. Interventions: Freshly sampled whole sheep ovaries were collected and prepared for histological analysis. Main Outcome Measure(s): The follicular densities and distributions were determined for hematoxylin and eosin sections. A mathematical model was derived based on the follicle counts and Monte-Carlo simulations. Results: Heterogeneous distributions and densities of primordial follicles were identified 1) for distinct areas of the same ovarian cortex, 2) between the ovaries of the same animal and 3) across different ewes. A mathematical model based on the analysis of 37,153 primordial follicles from 8 different ovaries facilitated the estimation of the number of cortical biopsies and sections that had to be analyzed to overcome such heterogeneity. Conclusion: The influence of physiological follicular heterogeneity on experimental and clinical results can be overcome when a definite number of cortical pieces and sections are taken into consideration.
Disciplines :
Reproductive medicine (gynecology, andrology, obstetrics)
Author, co-author :
Fransolet, Maïté ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Labied, Soraya ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Henry, Laurie  ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Masereel, Marie-Caroline
Rozet, Eric ;  Université de Liège - ULiège > Département de pharmacie > Chimie analytique
Kirschvink, Nathalie
Nisolle, Michelle ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Munaut, Carine  ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Language :
English
Title :
Strategies for Using the Sheep Ovarian Cortex as a Model in Reproductive Medicine
Publication date :
2014
Journal title :
PLoS ONE
eISSN :
1932-6203
Publisher :
Public Library of Science, San Franscisco, United States - California
Volume :
9
Issue :
3
Pages :
e91073
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 11 April 2014

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