Reference : Phenotypic Characterization of Osteoblasts from the Sclerotic Zones of Osteoarthritic...
Scientific journals : Article
Human health sciences : Rheumatology
http://hdl.handle.net/2268/1647
Phenotypic Characterization of Osteoblasts from the Sclerotic Zones of Osteoarthritic Subchondral Bone
English
Sanchez, Christelle mailto [Université de Liège - ULg > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.) >]
Deberg, Michelle mailto [Université de Liège - ULg > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.) >]
Bellahcene, Akeila mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Labo de recherche sur les métastases >]
Castronovo, Vincenzo mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire >]
Msika, Philippe [>]
Delcour, Jean-Pierre [>]
Crielaard, Jean-Michel mailto [Université de Liège - ULg > Département des sciences de la motricité > Evaluation et entraînement des aptitudes physiques >]
Henrotin, Yves mailto [Université de Liège - ULg > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.) - Didactique des sciences de la santé - Pathologie générale et physiopathologie >]
Feb-2008
Arthritis and Rheumatism
58
2
442-55
Yes (verified by ORBi)
International
0004-3591
[en] Aged ; Alkaline Phosphatase/genetics/metabolism ; Cells, Cultured ; Collagen/genetics ; Collagen Type I/genetics ; Cytokines/genetics ; Extracellular Matrix Proteins/genetics ; Gene Expression/physiology ; Knee Joint/*pathology ; Matrix Metalloproteinase 13/genetics ; Osteoarthritis, Knee/genetics/*pathology/*physiopathology ; Osteoblasts/*pathology/*physiology
[en] OBJECTIVE: To determine the phenotype of osteoblasts from the sclerotic zones of human osteoarthritic (OA) subchondral bone. METHODS: Human osteoblasts were isolated from sclerotic or nonsclerotic areas of subchondral bone and cultured for 14 days in monolayer. The expression of 14 genes was investigated by real-time reverse transcription-polymerase chain reaction. The activities of alkaline phosphatase (AP) and transglutaminases (TGases) were quantified by enzymatic assays. C-terminal type I procollagen propeptide (CPI), interleukin-1beta (IL-1beta), IL-6, IL-8, transforming growth factor beta1 (TGFbeta1), osteocalcin (OC), and osteopontin (OPN) were assayed in the culture medium by immunoassay. RESULTS: Gene expression levels of matrix metalloproteinase 13, COL1A1 and COL1A2, OPN, tissue-nonspecific AP, OC, vascular endothelial growth factor, ANKH, TGase 2, factor XIIIA, and dentin matrix protein 1 were significantly up-regulated in sclerotic osteoblasts compared with nonsclerotic osteoblasts. In contrast, parathyroid hormone receptor gene expression was depressed in sclerotic osteoblasts, but bone sialoprotein levels were unchanged. The activities of AP and TGases were increased in sclerotic osteoblasts, while matrix mineralization, revealed by alizarin red staining, was decreased. In parallel, protein synthesis of CPI, OC, OPN, IL-6, IL-8, and TGFbeta1 was significantly higher in sclerotic osteoblasts than in nonsclerotic osteoblasts, while IL-1beta production was similar in both groups. CONCLUSION: These findings contribute to a better understanding of the mechanisms involved in subchondral bone sclerosis and identify osteoblasts with an altered phenotype as a potential target for future OA therapies.
http://hdl.handle.net/2268/1647
10.1002/art.23159

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