Improvement of fermentative biohydrogen production by Clostridium butyricum CWBI1009 in sequenced-batch, horizontal fixed bed and biodisc-like anaerobic reactors with biomass retention
Clostridium; hydrogen production; Biofilm; Bioreactor; Pure strain
Abstract :
[en] A horizontal tubular fixed bed bioreactor (HFBR) and an anaerobic biodisc-like reactor (AnBDR) were designed to both fix Clostridium biomass and enable rapid transfer of the hydrogen produced to gas phase in order to decrease the strong effect of H2 partial pressure and H2 supersaturation on the performances of Clostridium strains. The highest H2 production rate (703 mL H2/L.h) and yield (302 mL/g glucose consumed i.e. 2.4 mol/mol) with the pure culture were recorded in the AnBDR with 300 mL culture medium (total volume 2.3 L) at pH 5.2 and a glucose loading rate of 2.87 g/L.h. These results are about 2.3 and 1.3-fold higher than those achieved in the same bioreactor with 500 mL liquid medium and with the same glucose consumption rate. Therefore, our experimentations and a short review of the literature reported in this paper emphasize the relevance of performing bioreactors with high L/G transfer.
Research center :
Centre Wallon de Biologie Industrielle
Disciplines :
Microbiology Biotechnology Chemical engineering
Author, co-author :
Hiligsmann, Serge ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Beckers, Laurent; Université de Liège - ULiège > Département des Sciences de la vie > Biochimie et microbiologie industrielles
Masset, Julien; Université de Liège - ULiège > Département des Sciences de la vie > Biochimie et microbiologie industrielles
Hamilton, Christopher; Université de Liège - ULiège > Département des Sciences de la vie > Biochimie et microbiologie industrielles
Thonart, Philippe ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Language :
English
Title :
Improvement of fermentative biohydrogen production by Clostridium butyricum CWBI1009 in sequenced-batch, horizontal fixed bed and biodisc-like anaerobic reactors with biomass retention
Etude de la production d'hydrogène par les bactéries anaérobies chimiotrophes (dark-fermentation)
Funders :
Action de Recherches concertées ARC 07/12 04- ULg- Communauté française FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE] F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] DGTRE - Région wallonne. Direction générale des Technologies, de la Recherche et de l'Énergie [BE]
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