[en] Freshwater snails of the family Lymnaeidae play an important role in the transmission of fascioliasis worldwide. In Vietnam, 2 common lymnaeid species, Lymnaea swinhoei and Lymnaea viridis, can be recognized on the basis of morphology, and a third species, Lymnaea sp., is known to exist. Recent studies have raised controversy about their role in transmission of Fasciola spp. because of confusion in identification of the snail hosts. The aim of this study is, therefore, to clarify the identities of lymnaeid snails in Vietnam by a combination of morphological and molecular approaches. The molecular analyses using the second internal transcribed spacer (ITS2) of the nuclear ribosomal DNA clearly showed that lymnaeids in Vietnam include 3 species, Austropeplea viridis (morphologically identified as L. viridis), Radix auricularia (morphologically identified as L. swinhoei) and Radix rubiginosa (morphologically identified as Lymnaea sp.). R. rubiginosa is a new record for Vietnam. Among them, only A. viridis was found to be infected with Fasciola spp. These results provide a new insight into lymnaeid snails in Vietnam. Identification of lymnaeid snails in Vietnam and their role in the liver fluke transmission should be further investigated.
Losson, Bertrand ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Caron, Yannick ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Language :
English
Title :
Morphological and Molecular Characterization of Lymnaeid Snails and Their Potential Role in Transmission of Fasciola spp. in Vietnam
Publication date :
December 2013
Journal title :
Korean Journal of Parasitology
ISSN :
0023-4001
eISSN :
1738-0006
Publisher :
Korean Society for Parasitology, Seoul, South Korea
Mas-Coma, M.S., Esteban, J.G., Bargues, M.D., Epidemiology of human fascioliasis: A review and proposed new classification (1999) Bull World Health Organ, 77, pp. 340-346
Trung, T.N., Chuong, N.V., Raising number of human fascioliasis cases in 2011 and demand for disease prevention at the community (2012) Annual Rep Instit Malariol Parasitol Entomol, , Quy Nhon, Vietnamese
Dalton, J.P., (1998) Fasciolosis, pp. 1-544. , Wallingford Oxon OX10 8DE, UK. CABI Publishing, CAB International
Correa, A.C., Escobar, J.S., Noya, O., Velásquez, L.E., Gonzáles-Ramírez, C., Hurtrez-Boussès, S., Poitier, J.P., Morphological and molecular characterization of neotropic Lymnaeidae (Gastropoda: Lymnaeoidea), vectors of fasciolosis (2011) Infect Genet Evol, 11, pp. 1978-1988
Hubendick, B., Recent Lymnaeidae, their variation, morphology, taxonomy, nomenclature and distribution (1951) Kungliga Svenska Vetenskapsakademiens Handlingar, pp. 1-35. , 17th ed, Stockkholm Almqvist and Wiksells Boktryckeri AB
Pfenninger, M., Cordellier, M., Streit, B., Comparing the efficacy of morphologic and DNA-based taxonomy in the freshwater gastropod genus Radix (Basommatophora, Pulmonata) (2006) BMC Evol Biol, 6, pp. 1-14
Thanh, D.N., Bai, T.T., Mien, P.V., (1980) Identification of Invertebrate In Northern Vietnam, pp. 230-250. , Ha Noi, Vietnam. Science and Technology Publishing House, Vietnamese
Correa, A.C., Escobar, J.S., Durand, P., Renaud, F., David, P., Jarne, P., Poitier, J.P., Hurtrez-Bousses, S., Bridging gaps in the molecular phylogeny of the Lymnaeidae (Gastropoda: Pulmonata), vectors of Fascioliasis (2010) BMC Evol Biol, 10, pp. 1-12
Doanh, P.N., Hien, H.V., Duc, N.V., Thach, D.T.C., New data on intermediate host of Fasciola in Vietnam (2012) J Biol, 34, pp. 139-144. , Vietnamese
Nguyen, S.T., Nguyen, D.T., Nguyen, T.V., Huyen, V.V., Le, D.Q., Fukuda, Y., Nakai, Y., Prevalence of Fasciola in cattle and of its intermediate host Lymnaea snails in central Vietnam (2012) Parasite, 19, pp. 85-89
Kim, N.T., Vinh, P.T., Investigation on the prevalence of fasciolosis in cattle and snails in Ha Bac midland and prevention method (1997) Annual Rep Instit Agricult Sci, Ha Noi, Vietnam, pp. 407-411. , Vietnamese
Ngai, D.D., Luc, P.V., Duc, N.V., Doanh, P.N., Ha, N.V., Minh, N.T., Animal raising practice and liver fluke prevalence in cattle in Daklak province (2006) J Biol, 8, pp. 68-72. , Vietnamese
Itagaki, T., Fujiwara, S., Mashima, K., Itagaki, H., Experimental infection of Japanese Lymnaea snails with Australian Fasciola hepatica (1988) Nihon Juigaku Zasshi, 50, pp. 1085-1091
Li, S., Xu, L., Li, L., Jiang, L., Yang, S., Yang, G., Lang, C., The experiment on artificial hatching and Radix swinhoei infection from Fasciola hepatica's eggs (2004) Yunnan Agricultural University, 19, pp. 360-364
Kaset, C., Eursithichai, V., Vichasri-Grams, S., Viyanant, V., Grams, R., Rapid identification of lymnaeid snails and their infection with Fasciola gigantica in Thailand (2010) Exp Parasitol, 126, pp. 482-488
Samadi, S., David, P., Jarne, P., Variation of shell shape in the clonal snail Melanoides tuberculata and its consequences for the interpretation of fossil series (2000) Evolution, 54, pp. 492-502
Burch, J.B., A guide to the freshwater snails of the Philippines (1980) Malacol Rev, 13, pp. 121-143
Shell, S.C., (1970) How to Know the Trematodes, pp. 1-35. , Dubuque, Iwoa, USA. WM C Brown Company Publisher
Caron, Y., Righi, S., Lempereur, L., Saegerman, C., Losson, B., An optimized DNA extraction and multiplex PCR for the detection of Fasciola sp. in lymnaeid snails (2011) Vet Parasitol, 178, pp. 93-99
Artigas, P., Bargues, M.D., Sierra, R.L.M., Agramunt, V.H., Mas-Coma, S., Characterisation of fascioliasis lymnaeid intermediate hosts from Chile by DNA sequencing, with emphasis on Lymnaea viator and Galba truncatula (2011) Acta Trop, 120, pp. 245-257
Thompson, J.D., Higgins, D.G., Gibson, T.J., CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice (1994) Nucleic Acids Res, 22, pp. 4673-4680
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S., Mega Molecular Evolotionary Genetics Analysis, , http://www.megasoftware.net, copyright 1993-2013. Available from
Puslednik, L., Ponder, W.F., Dowton, M., Davis, A.R., Examining the phylogeny of the Australasian Lymnaeidae (Heterobranchia: Pulmonata: Gastropoda) using mitochondrial, nuclear and morphological markers (2009) Mol Phylogenet Evol, 52, pp. 643-659
Cipriana, P., Mattiucci, S., Paoletti, M., Scialana, F., Nascetti, G., Molecular evidence of Trichobilharzia franki Muller and Kimmig, 1994 (Digenea: Schistosomatidae) in Radix auricularia from Central Italy (2011) Parasitol Res, 109, pp. 935-940
Ferté, H., Depaquit, J., Carré, S., Villena, I., Léger, N., Presence of Trichobilharzia szidati in Lymnaea stagnalis and T. franki in Radix auricularia in northeastern France: Molecular evidence (2005) Parasitol Res, 95, pp. 150-154
Huňová, K., Kašný, M., Hampl, V., Leontovyč, R., Kuběna, A., Mikeš, L., Horák, P., Radix spp.: Identification of trematode intermediate hosts in the Czech Republic (2012) Acta Parasitol, 57, pp. 273-284
Durand, P., Poitier, J.P., Escoubeyrou, K., Arenas, J.A., Yong, M., Amarista, M., Bargues, M.D., Renaud, F., Occurrence of a sibling species complex within neotropical lymnaeids, snail intermediate hosts of fascioliasis (2002) Acta Trop, 83, pp. 233-240
Bargues, M.D., Mas-Coma, S., Reviewing lymnaeid vectors of fascioliasis by ribosomal DNA sequence analyses (2005) J Helminthol, 79, pp. 257-267
Lan, P.D., Biological characteristic of Fasciola gigantica and fasciolosis in buffalo in northern Vietnam (1983) J Sci Technol, 12, pp. 675-678. , Vietnamese
