Article (Scientific journals)
Syntenin, a syndecan adaptor and an Arf6 phosphatidylinositol 4,5-bisphosphate effector, is essential for epiboly and gastrulation cell movements in zebrafish.
Lambaerts, Kathleen; Van Dyck, Stijn; Mortier, Eva et al.
2012In Journal of Cell Science, 125 (Pt 5), p. 1129-40
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Keywords :
ADP-Ribosylation Factors/metabolism; Animals; Cell Movement/physiology; Cytoskeleton/genetics; Gastrulation/physiology; Gene Knockdown Techniques; Mice; Molecular Sequence Data; Phosphatidylinositol 4,5-Diphosphate/metabolism; Syndecans/metabolism; Syntenins/genetics/metabolism; Zebrafish/embryology/metabolism/physiology; Zebrafish Proteins/genetics/metabolism
Abstract :
[en] Epiboly, the spreading and the thinning of the blastoderm to cover the yolk cell and close the blastopore in fish embryos, is central to the process of gastrulation. Despite its fundamental importance, little is known about the molecular mechanisms that control this coordinated cell movement. By a combination of knockdown studies and rescue experiments in zebrafish (Danio rerio), we show that epiboly relies on the molecular networking of syntenin with syndecan heparan sulphate proteoglycans, which act as co-receptors for adhesion molecules and growth factors. Furthermore, we show that the interaction of syntenin with phosphatidylinositol 4,5-bisphosphate (PIP2) and with the small GTPase ADP-ribosylation factor 6 (Arf6), which regulate the endocytic recycling of syndecan, is necessary for epiboly progression. Analysis of the earliest cellular defects suggests a role for syntenin in the autonomous vegetal expansion of the yolk syncytial layer and the rearrangement of the actin cytoskeleton in extra-embryonic tissues, but not in embryonic cell fate determination. This study identifies the importance of the syntenin-syndecan-PIP2-Arf6 complex for the progression of fish epiboly and establishes its key role in directional cell movements during early development.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Lambaerts, Kathleen
Van Dyck, Stijn
Mortier, Eva
Ivarsson, Ylva
Degeest, Gisele
Luyten, Annouck
Vermeiren, Elke
Peers, Bernard ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
David, Guido
Zimmermann, Pascale
Language :
English
Title :
Syntenin, a syndecan adaptor and an Arf6 phosphatidylinositol 4,5-bisphosphate effector, is essential for epiboly and gastrulation cell movements in zebrafish.
Publication date :
2012
Journal title :
Journal of Cell Science
ISSN :
0021-9533
eISSN :
1477-9137
Publisher :
The Company of Biologists, United Kingdom
Volume :
125
Issue :
Pt 5
Pages :
1129-40
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 08 March 2014

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