[en] A lipase gene (lip1) from the facultative psychrophilic strain Psychrobacter immobilis B10 has been cloned and sequenced. The deduced preprotein sequence is composed of 317 amino acids with a predicted M(r) of 35,288. A primary structure alignment of lipases including lip1 shows conserved elements for which a structural role is proposed in the light of recent crystallographic studies. The analysis of the psychrophilic enzyme sequence suggests characteristics in relation with the adaptation to cold.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Arpigny, J. L.
Feller, Georges ; Université de Liège - ULiège > Département des sciences de la vie > Labo de biochimie
Gerday, Charles ; Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)
Language :
English
Title :
Cloning, Sequence and Structural Features of a Lipase from the Antarctic Facultative Psychrophile Psychrobacter Immobilis B10
Merkler, Farrington, Wedler Int. J. Pept. Prot. Res. 1981, 18:430-434.
Menendez-Arias, Argos J. Mol. Biol. 1989, 206:397-406.
Mrabet, Van den Broeck, Van den Brande, Stanssens, Laroche, Lambeir, Matthijssens, Jenkins, Chiadmi, Van Tilbeurgh, Rey, Janin, Quax, Lasters, De Maeyer, Wodak Biochemistry 1992, 31:2239-2253.
Wicker-Planquart, Puigserver FEBS Lett. 1992, 296:61-66.
