[en] Size of methyl ricinoleate droplets during biotransformation into γ-decalactone by Yarrowia lipolytica was measured in both homogenized and non-homogenized media. In non-homogenized but shaken medium, droplets had an average volume surface diameter d 32 of 2.5 μm whereas it was 0.7 μm in homogenized and shaken medium. But as soon as yeast cells were inoculated, both diameters became similar at about 0.7 μm and did not vary significantly until the end of the culture. The growth of Y. lipolytica in both media was very similar except for the lag phase which was lowered in homogenized medium conditions.
Disciplines :
Biotechnology
Author, co-author :
Waché, Y.; Laboratoire de Biotechnologie, ENSBANA-Universite de Bourgogne, Dijon, France, Lab. de Biotechnologie ENSBANA, I, esplanade Erasme, 21000 Dijon, France
Bergmark, K.; Laboratoire de Biotechnologie, ENSBANA-Universite de Bourgogne, Dijon, France
Courthaudon, J.-L.; Lab. de Biochimie alimentaire, ENSBANA-Universite de Bourgogne, Dijon, France
Aguedo, Mario ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Nicaud, J.-M.; Lab. de Genetique des M., INRA-CNRS, Thiverval-Grignon, France
Belin, J.-M.; Laboratoire de Biotechnologie, ENSBANA-Universite de Bourgogne, Dijon, France
Language :
English
Title :
Medium-size droplets of methyl ricinoleate are reduced by cell-surface activity in the γ-decalactone production by Yarrowia lipolytica
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