[en] Some microorganisms can transform methyl ricinoleate into γ-decalactone, a valuable aroma compound, but yields of the bioconversion are low due to (i) incomplete conversion of ricinoleate (C18) to the C10 precursor of γ-decalactone, (ii) accumulation of other lactones (3-hydroxy-γ-decalactone and 2- and 3-decen-4-olide), and (iii) γ-decalactone reconsumption. We evaluated acyl coenzyme A (acyl-CoA) oxidase activity (encoded by the POX1 through POX5 genes) in Yarrowia lipolytica in lactone accumulation and γ-decalactone reconsumption in POX mutants. Mutants with no acyl-CoA oxidase activity could not reconsume γ-decalactone, and mutants with a disruption of pox3, which encodes the short-chain acyl-CoA oxidase, reconsumed it more slowly. 3-Hydroxy-γ-decalactone accumulation during transformation of methyl ricinoleate suggests that, in wild-type strains, β-oxidation is controlled by 3-hydroxyacyl-CoA dehydrogenase. In mutants with low acyl-CoA oxidase activity, however, the acyl-CoA oxidase controls the β-oxidation flux. We also identified mutant strains that produced 26 times more γ-decalactone than the wild-type parents.
Disciplines :
Biotechnology
Author, co-author :
Waché, Y.; Laboratoire de Biotechnologie, Ecl. Natl. Sup. Biol. Appl. Nutr. A., Université de Bourgogne, 21000 Dijon, France, Laboratoire de Biotechnologie, Equipe IMSA, ENSBANA, 1, Esplanade Erasme, 21000 Dijon, France
Aguedo, Mario ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Choquet, A.; Laboratoire de Biotechnologie, Ecl. Natl. Sup. Biol. Appl. Nutr. A., Université de Bourgogne, 21000 Dijon, France
Gatfield, I. L.; Haarmann and Reimer GmbH, Flavour Research, 37601 Holzminden, Germany
Nicaud, J.-M.; Lab. de Genet. des Micro-organismes, INRA-CNRS, URA1925, 78850 Thiverval-Grignon, France
Belin, J.-M.; Laboratoire de Biotechnologie, Ecl. Natl. Sup. Biol. Appl. Nutr. A., Université de Bourgogne, 21000 Dijon, France
Language :
English
Title :
Role of β-Oxidation Enzymes in γ-Decalactone Production by the Yeast Yarrowia lipolytica
Publication date :
2001
Journal title :
Applied and Environmental Microbiology
ISSN :
0099-2240
eISSN :
1098-5336
Publisher :
American Society for Microbiology, Washington, United States - District of Columbia
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