Genetic engineering of the β-oxidation pathway in the yeast Yarrowia lipolytica to increase the production of aroma compounds

Groguenin, A.; Waché, Y.; Garcia, E. E.; Aguedo, Mario; Husson, F.; LeDall, M.-T.; Nicaud, J.-M.; Belin, J.-M.

doi:10.1016/j.molcatb.2004.01.006

Article (Scientific journals)

Genetic engineering of the β-oxidation pathway in the yeast Yarrowia lipolytica to increase the production of aroma compounds

Groguenin, A.; Waché, Y.; Garcia, E. E. et al.

2004 • In Journal of Molecular Catalysis B: Enzymatic, 28 (2-3), p. 75-79

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/162763

DOI
10.1016/j.molcatb.2004.01.006

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Keywords :

Acyl-CoA oxidase; Hydroxy acids; Biodegradation; Catalysis; Enzymes; Genetic engineering; Oxidation; Lactone; Yeast strain; Yeast; Yarrowia lipolytica

Abstract :

[en] The yeast Yarrowia lipolytica possesses five acyl-CoA oxidases (Aox1p to 5), the enzyme catalysing the first reaction of β-oxidation. The understanding of the specific role of each acyl-CoA oxidase is important to construct a yeast strain growing at a good rate and able to produce without degrading the aroma compound γ-decalactone. In this study we observed that Aox4p exhibits a slight activity on a broad spectrum of substrates and that it is involved in lactone degradation. We constructed a strain lacking this activity. Its growth was only slightly altered and it produced 10 times more lactone than the wild type in 48h. © 2004 Elsevier B.V. All rights reserved.

Disciplines :

Biotechnology

Author, co-author :

Groguenin, A.; Lab. Microbiologie UMR UB-INRA 1232, ENSBANA, 1, Esplanade Erasme, 21000 Dijon, France

Waché, Y.; Lab. Microbiologie UMR UB-INRA 1232, ENSBANA, 1, Esplanade Erasme, 21000 Dijon, France

Garcia, E. E.; Lab. Microbiologie UMR UB-INRA 1232, ENSBANA, 1, Esplanade Erasme, 21000 Dijon, France

Aguedo, Mario ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle

Husson, F.; Lab. Microbiologie UMR UB-INRA 1232, ENSBANA, 1, Esplanade Erasme, 21000 Dijon, France

LeDall, M.-T.; Lab. Microbiologie et Genet. Molec., INRA-CNRS-INAPG UMR 2585, 78850 Thiverval-Grignon, France

Nicaud, J.-M.; Lab. Microbiologie et Genet. Molec., INRA-CNRS-INAPG UMR 2585, 78850 Thiverval-Grignon, France

Belin, J.-M.; Lab. Microbiologie UMR UB-INRA 1232, ENSBANA, 1, Esplanade Erasme, 21000 Dijon, France

Language :

English

Title :

Genetic engineering of the β-oxidation pathway in the yeast Yarrowia lipolytica to increase the production of aroma compounds

Publication date :

2004

Journal title :

Journal of Molecular Catalysis B: Enzymatic

ISSN :

1381-1177

eISSN :

1873-3158

Publisher :

Elsevier, Netherlands

Volume :

Issue :

2-3

Pages :

75-79

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 05 February 2014

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