Partial characterization of testosterone-metabolizing enzymes in the quail brain.

3-Oxo-5-alpha-Steroid 4-Dehydrogenase/metabolism; Animals; Aromatase/metabolism; Brain/enzymology; Coturnix/metabolism; Dihydrotestosterone/biosynthesis; Estradiol/biosynthesis; Kinetics; Oxidoreductases/metabolism; Quail/metabolism; Temperature; Testosterone/metabolism

Abstract :

[en] The properties of 5 beta-reductase, 5 alpha-reductase and aromatase, 3 testosterone metabolizing enzymes, were studied in the quail brain by an in vitro incubation technique. The results describe the changes in time of metabolite production and the effects of temperature, enzyme and cofactor concentrations. The apparent Km and Vmax were evaluated for the 3 enzymes. Aromatase and 5 alpha-reductase have a higher affinity but a lower capacity than 5 beta-reductase. The kinetics of the latter enzyme are complex and suggest the presence of two types of enzymes. These characteristics fit in well with the role probably played by the enzymes in vivo.

Disciplines :

Zoology
Neurosciences & behavior

Author, co-author :

Schumacher, M.

Contenti, E.

Balthazart, Jacques ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie de la différenciation sexuelle du cerveau

Language :

English

Title :

Partial characterization of testosterone-metabolizing enzymes in the quail brain.

Publication date :

1984

Journal title :

Brain Research

ISSN :

0006-8993

eISSN :

1872-6240

Publisher :

Elsevier, Netherlands

Volume :

305

Issue :

Pages :

51-9

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 05 February 2014

Statistics

Number of views

47 (1 by ULiège)

Number of downloads

1 (1 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

Bibliography

Adkins, Boop, Koutnik, Morris, Pniewski (1980) Further evidence that androgen aromatization is essential for the activation of copulation in male quail. Physiol. Behav 24:441-446.
Balthazart (1983) Hormonal correlates of behavior. Avian Biology, 7 , D.S. Farner, J.R. King, K.C. Parkes, Academic Press, New York; 221-365.
Balthazart, Massa, Negri-Cesi (1979) Photoperiodic control of testosterone metabolism, plasma gonadotrophins, cloacal gland growth and reproductive behaviour in the Japanese quail. Gen. comp. Endocr. 39:222-235.
Balthazart, Schumacher, Ottinger (1983) Sexual differences in the Japanese quail: behavior, morphology and intracellular metabolism of testosterone. Gen. comp. Endocr. 51:191-207.
.
Callard, Petro, Ryan (1978) Conversion of androgen to estrogen and other steroids in the vertebrate brain. Amer. Zool. 18:511-523.
Callard, Petro, Ryan (1979) In vitro aromatization and other androgen transformation in the brain of the hamster (Mesocricetus auratus). Biol. Reprot. 21:33-38.
Challis, Harrison, Heap (1973) The kinetics of estradiol-17β metabolism in the sheep. J. Endocr. 57:97-110.
Davies, Massa, James (1980) Role of testosterone and of its metabolites in regulating gonadotrophin secretion in the Japanese quail. J. Endocr. 84:221-222.
Feder (1978) Specificity of steroid hormone activation of sexual behaviour in rodents. Biological Determinants of Sexual Behavior , J.B. Hutchison, Wiley, New York; 395-424.
Hess, Lees, Derr (1978) A linear Lowry-Folin assay for water-soluble and sodium dodecyl sulfatesolubilized proteins. Analyt. Biochem. 85:295-300.
Hutchison, Steimer (1981) Brain 5β-reductase. A correlate of behavioral sensitivity to androgen. Science 213:244-246.
Hutchison, Steimer (1983) Hormone-mediated behavioural transitions: a role for brain aromatase. Hormones and Behaviour in Higher Vertebrates , J. Balthazart, E. Prove, R. Gilles, Springer, New York; 261-274.
Hutchison, Steimer, Duncan (1981) Behavioural action of androgen in the dove: effects of long-term castration on response specificity and brain aromatization. J. Endocr. 90:167-178.
Jouan, Samperez (1980) Metabolism of steroid hormones in the brain. The Endocrine Functions of the Brain , M. Motta, Raven Press, Heidelberg; 95-115.
Kawahara, Berman, Green (1975) Conversion of progesterone-1,2-3H to 5β-pregnane-3,20-dione by brain tissue. Steroids 25:459-463.
Lisboa, Strassner, Wulff, Hoffmann (1974) 5β-reductase in the human fetal brain. Acta endocr. , (abstract); 156.
Luttge (1979) Endocrine control of mammalian male sexual behavior: an analysis of the potential role of testosterone metabolites. Endocrine Control of Sexual Behavior , C. Beyer, Raven Press, New York; 341-363.
Martini (1982) The 5α-reduction of testosterone in the neuroendocrine structures. Biochemical and physiological implications. Endocr. Rev. 3:1-25.
Massa, Bottoni, Lucini (1983) Brain testosterone metabolism and sexual behaviour in birds. Hormones and Behaviour in Higher Vertebrates , J. Balthazart, E. Prove, R. Gilles, Springer, New York; 230-236.
Massa, Cresti, Martini (1977) Metabolism of testosterone in the anterior pituitary gland and the central nervous system of the European starling (Sturnus vulgaris). J. Endocr. 75:347-354.
Massa, Davies, Bottoni, Martini (1979) Photoperiodic control of steroid production and action. J. Steroid. Biochem. 11:937-944.
Nakamura, Tanabe (1974) In vitro metabolism of steroid hormones by chicken brain. Acta Endocr. 75:410-416.
Nguyen, Duval, Samperez, Jouan (1974) Testosterone 5α-reductase of microsomes from rat anterior hypophysis: properties, increase by castration and hormonal control. Biochimie 56:899-906.
Ricketts, Galil, Ackland, Heap, Flint (1980) Activation by corticosteroids of steroid metabolizing enzymes in ovine placental explants in vitro. J. Endocr. 85:457-469.
Schumacher, Balthazart (1983) The effects of testosterone and its metabolites on sexual behavior and morphology in male and female Japanese quail. Physiol. Behav. 30:335-339.
.
Schumacher, Contenti, Balthazart (1983) Testosterone metabolism in discrete areas of the hypothalamus and adjacent brain regions of male and female Japanese quail (Coturnix coturnix japonica). Brain Research 278:337-340.
Selmanoff, Brodkin, Weiner, Siiteri (1977) Aromatization and 57ga-reduction of androgens in discrete hypothalamic and limbic regions of the male and female rat. Endocrinology 101:841-848.
Steimer, Hutchison (1981) Metabolic control of the behavioral action of androgens in the dove brain: testosterone inactivation by 5β-reduction. Brain Research 209:189-204.
Steimer, Hutchison (1981) Androgen increases formation of behaviourally effective oestrogen in the dove brain. Nature (Lond.) 292:345-347.
Van Dorn, Clark (1973) In vitro studies on the inhibition of pig liver steroid delta4-5Î²-reductase activity by naturally occurring and synthetic estrogens. Biochimica et Biophysica Acta (BBA) - Enzymology 309:254-262.
Wingfield, Farner (1975) The determination of five steroids in avian plasma by radioimmunoassay and competitive protein binding. Steroids 26:315-327.
Wingfield, Farner (1980) Control of seasonal reproduction in temperate-zone birds. Progr. Reprod. Biol. 5:62-101.