[en] A Gas Chromatography-Flame Ionization Detector method for characterization and
micro-quantification of sterols was developed. The applicability of this method was evaluated
for determining sterolic compounds in pollen loads, larvae, and adults of the honeybee.
After a multi-step procedure to extract and purify sterols, the compounds were identified on
the basis of their retention data and quantified on the basis of peak areas from analyses.
Quantifications were feasible with a limited amount of samples, allowing single specimen
analyses and estimation of inter-individual variation. The reliability of this microquantitative
method for sterol analysis was also investigated.
Disciplines :
Chemistry Entomology & pest control
Author, co-author :
Vanderplanck, Maryse ; University of Mons > Research Institute for Biosciences > Laboratory of Zoology
Michez, Denis; Université de Mons - UMONS
Vancraenenbroeck, Sophie ; Université de Liège - ULiège > Chimie et bio-industries > Chimie analytique
Lognay, Georges ; Université de Liège - ULiège > Chimie et bio-industries > Chimie analytique
Language :
English
Title :
Micro-quantitative method for analysis of sterol levels in honeybees and their pollen loads
Publication date :
June 2011
Journal title :
Analytical Letters
ISSN :
0003-2719
eISSN :
1532-236X
Publisher :
Marcel Dekker, New York, United States - New York
Volume :
44
Issue :
10
Pages :
1807-1820
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
A nonymous. 1983. Corps gras d'origine animale et végétale. Dosage des faibles teneurs en Cholestérol. Norme française. NF T 60-249.
Behmer, S. T., and W. D. Nes. 2003. Insect sterol nutrition and physiology: A global overview. Adv. Insect Physiol. 31: 1-72.
Hobson, R. P. 1935. On a fat-soluble growth factor required by blowfly larvae. II. Identity of the growth factor with cholesterol. Biochem. J. 29: 2023-2026.
IUPAC. 1987. Standard methods for the analysis of oils, fats and derivaties, method 2, 7th ed. Oxford, UK: Blackwell.
Kircher, H. W., W. B. Heed, J. S. Russell, and J. Groove. 1967. Senita cactus alkaloids: Their significance to Sonoran desert Drosophila ecology. J. Insect Physiol. 13: 1869-1874.
Lognay, G., A. Boenke, P. J. Wagstaffe, and M. Severin. 1992. Edible fats and oils reference materials for sterol analysis with particular attention to cholesterol. Part I. Investigation of some analytical aspects by experienced laboratories. Analyst 117: 1093-1097.
Lognay, G., P. Dreze, P. J. Wagstaffe, M. Marlier, and M. Severin. 1989. Validation of a quantitative procedure for the extraction of sterols from edible oils using radiolabelled compounds. Analyst 114: 1287-1291.
Mondy, N., and M.-F. Corio-Costet. 2000. The response of the grape berry moth (Lobesia botrana) to a dietary phytopathogenic fungus (Botrytis cinerea): The significance of fungus sterols. J. Insect Physiol. 46: 1557-1564.
Nation, J. L. 2002. Insect physiology and biochemistry.Boca Raton, FL: CRC Press LLC.
Norris, D. M., and J. K. Baker. 1967. Symbiosis: Effects of a mutualistic fungus upon the growth and reproduction of Xyleborus ferrugineus. Science 156: 1120-1122.
Osswald, W. F., W. Hö ll, and E. F. Elstner. 1986. Ergosterol as a biochemical indicator of fungal infection in spruce and fir needles from different sources.Z.Naturforsch. 41(c): 542-546.
Rasmont, P., A. Regali, T. C. Ings, G. Lognay, E. Baudart, M. Marlier, E. Delcarte, et al, 2005. Analysis of pollen and nectar of Arbutus unedo as a food source for Bombus terrestris (Hymenoptera: Apidae). J. Econ. Entomol. 98: 656-663.
Regali, A. 1996. Contribution al'étude des besoins alimentaires en stéroi{dotless}̈des de Bombus terrestris (L.). Ph.D. Dissertation in Biology. Laboratory of Zoology, Université de Mons-Hainaut.
Schwadorf, K., and H.-M. Müller. 1989. Determination of ergosterol in cereals, mixed feed components, and mixed feeds by liquid chromatography. J. Assoc. Offic. Anal. Chem. 72: 457-462.
Svoboda, J. A., and M. F. Feldlaufer. 1991. Neutral sterol metabolism in insects. Lipids 26: 614-618.
Svoboda, J. A., M. J. Thompson, E. W. Jr. Herbert, and H. Shimanuki. 1980. Sterol utilization in honey bees fed a synthetic diet: Analysis of prepupal sterols. J. Insect Physiol. 26: 291-294.
Svoboda, J. A., G. F. Weirich, and M. F. Feldlaufer. 1991. Recent advances in insect steroid biochemistry. In Physiology and biochemistry of sterols, ed. G. W. Patterson and W. D. Nes. Champaign, IL: American Oil Chemist's Society.
