[en] The complex microbial community living in the hindgut of lower termites includes prokaryotes, flagellates, yeasts, and filamentous fungi. Many microorganisms are found in the termite gut, but only a few are thought to be involved in symbiotic association to participate in cellulose digestion. Proteomics provides analyses from both taxonomical and functional perspectives. We aimed to identify symbiont diversity in the gut of Reticulitermes santonensis (Feytaud), via complementary electrospray ionization associated to ion trap tandem mass spectrometry (LC-MS/MS) and two-dimensional gel electrophoresis associated to matrix-assisted laser desorption-ionization-time-of-flight mass spectrometry analysis. One specific challenge to the study of lower termites is the relatively few data available on abundant symbiotic flagellates. Analysis based on LC-MS/MS revealed few protein families showing assignments to eukaryotes and the taxonomic origin of highly represented actins could not be established. Tubulins proved to be the most suitable protein family with which to identify flagellate populations from hindgut samples using LC-MS/MS, compared with other protein families, although this method targeted few prokaryotes in our assay. Similarly, two-dimensional gel electrophoresis associated to matrix-assisted laser desorption-ionization-time-of-flight mass spectrometry did not succeed in identifying flagellate populations, but did permit the identification of most of the prokaryotic components of the symbiotic system. Finally, fungi and yeasts were identified by both methods. Owing to the lack of sequenced genes in flagellates, targeting tubulins for LC-MS/MS could allow fingerprints of flagellate populations to be established. Experimental and technical improvements might increase the efficiency of identification of prokaryotic populations in the near future, based on metaproteomic development.
Disciplines :
Biotechnology
Author, co-author :
Bauwens, Julien ; Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Millet, Catherine ; Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique
Tarayre, Cédric ; Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Brasseur, Catherine ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Destain, Jacqueline ; Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Vandenbol, Micheline ; Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique
Thonart, Philippe ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Portetelle, Daniel ; Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique
De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Haubruge, Eric ; Université de Liège - ULiège > Vice-Recteur de Gembloux Agro Bio Tech
Francis, Frédéric ; Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Language :
English
Title :
Symbiont Diversity in Reticulitermes santonensis (Isoptera: Rhinotermitidae): Investigation Strategy Through Proteomics.
Publication date :
2013
Journal title :
Environmental Entomology
ISSN :
0046-225X
eISSN :
1938-2936
Publisher :
Entomological Society of America, United States - Maryland
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