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Proteomic study of lumbar spinal cord after quadricipital eccentric exercise
Lacrosse, Zoé; Hody, Stéphanie; Leprince, Pierre et al.
2013Euron PhD Day
 

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Keywords :
Lumbar spinal cord; Eccentric exercise; Proteomic
Abstract :
[en] Eccentric muscle contractions are characterized by an increase of muscle tension as it lengthens (slowering movements). Unaccustomed or intense eccentric exercise causes “Delayed-Onset Muscle Soreness” (DOMS). DOMS include muscle pain that appears 24 to 72 hours after exercise, but also stiffness, edema and muscle proteins release in plasma as a hallmark of muscle fibers injuries. The only systematic intervention that brings a muscle protection against DOMS is to realize submaximal eccentric contractions with a progressively increased intensity. The mechanism of this protection, called the “Repeated Bout Effect” (RBE), is not understood. However, it is likely explained by cellular, mechanical and neural theories [Scand.J.Med.&Sci.Sports, 13, 88, 2003]. The objective of this study is to better understand which neural signal is released in the muscle synapse and which brings protection by RBE. Male adult mice (C57BL6) were randomly divided into downhill running (DHR), uphill running (UHR) and untrained control (CONT) groups (n=4/group). DHR group is characterized by eccentric contractions of the quadriceps while UHR is concerned by concentric contractions. Running groups performed a warm-up of ten minutes followed by an interval exercise on an inclined treadmill at a velocity of 20cm/s. The latter consisted of running 18 bouts of 5 minutes interspersed with a 2 minutes rest. Lumbar spinal cord was dissected 24h after the race. Nuclear and cytoplasmic proteins were separately extracted and subjected to a 2D-DIGE analysis coupled with mass spectrometry. We do not observe any cytoplasmic protein modification while in the nuclear extract, seven spots were more abundant in eccentric group and four in concentric group in comparison with control group. The mass spectrometry of these proteins reveals that they are implicated in axoplasmic transport. At 24 hours, too few proteins modifications were detected in lumbar spinal cord, maybe as a consequence of a too short period between race and euthanasia. Implication of axoplasmic transport comforts our starting hypothesis that nervous system is able to protect muscle during the RBE by a synthesis and then a synaptic release of molecules modifying the muscle physiology.
Research center :
Giga-Neurosciences - ULiège
Disciplines :
Neurology
Author, co-author :
Lacrosse, Zoé ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Hody, Stéphanie ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Leprince, Pierre ;  Université de Liège - ULiège > GIGA - Neurosciences
Croisier, Jean-Louis ;  Université de Liège - ULiège > Département des sciences de la motricité > Kinésithérapie générale et réadaptation
Rogister, Bernard  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Language :
English
Title :
Proteomic study of lumbar spinal cord after quadricipital eccentric exercise
Alternative titles :
[fr] Etude protéomique de la moelle épinière lombaire après un exercice eccentrique quadricipital
Publication date :
12 September 2013
Number of pages :
A0
Event name :
Euron PhD Day
Event organizer :
Ulg Giga-Neurosciences
Event place :
Liège, Belgium
Event date :
du 12 septembre 2013 au 13 septembre 2013
Audience :
International
Name of the research project :
Role of nervous system in muscle typology remodeling induce by eccentric exercise
Available on ORBi :
since 27 January 2014

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