Poster (Scientific congresses and symposiums)
Microfluidic on optical fibers: Towards a new kind of fluorescent biosensor
Lismont, Marjorie; Vandewalle, Nicolas; Weyer, Floriane et al.
2014SPIE West 2014
 

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Abstract :
[en] In recent works, the behavior of droplets moving along vertical treads due to gravity was studied. It appeared that the droplet can be stopped by encountering a horizontal fiber depending on droplet volumes and fiber characteristics. On the basis of this behavior and by replacing treads by two crossed optical fibers, it is possible to combine fluidics and optics to develop a new kind of fluorescent sensor. In our work, the intersection between two crossed optical fibers is used as the basic unit of an original optofluidic biosensor. These two optical fibers are used as droplets carriers: one for probe molecules and the other one for target species. The fiber's junction catches the droplets and act as a reaction center. The main advantage of using optical fibers resides in their ability to propagate and collect light to and from the droplet localized at the fiber's crossing. This optical fiber configuration can therefore allow the study of biological interactions using fluorescent labels. This new and versatile detection scheme was validated on a calcium indicator where ions detection is accomplished by using a dye, Oregon green Bapta-2, that has a Ca 2+ recognition group as well as an entity exhibiting fluorescence. A FRET recognition event, between Rh-Con A and FITC-Dextran, was also investigated to detect glucose. Finally, a prototype of a multiplexing device, composed of several juxtaposed fibers' junctions, was developed.
Disciplines :
Physics
Author, co-author :
Lismont, Marjorie ;  Université de Liège - ULiège > Département de physique > Biophotonique
Vandewalle, Nicolas  ;  Université de Liège - ULiège > Département de physique > Physique statistique
Weyer, Floriane ;  Université de Liège - ULiège > Département de physique > Physique statistique
Joris, Bernard ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes
Dreesen, Laurent ;  Université de Liège - ULiège > Département de physique > Biophotonique
Language :
English
Title :
Microfluidic on optical fibers: Towards a new kind of fluorescent biosensor
Publication date :
February 2014
Number of pages :
A1
Event name :
SPIE West 2014
Event place :
San Francisco, United States
Event date :
du 1 février 2014 au 6 février 2014
Audience :
International
Available on ORBi :
since 24 January 2014

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