Reference : Effects of constitutively active GTPases on fibroblast behavior.
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/16167
Effects of constitutively active GTPases on fibroblast behavior.
English
Zhang, Z.-G. [>University of Cologne > > > > Center for Biochemistry, Center for Molecular Medecine > >]
Lambert, Charles mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs > > > >]
Servotte, S. [>Université de Liège > > >Département des sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs > >]
Chometon, G. [>University of Cologne > > > > Center for Biochemistry > >]
Eckes, B. [>University of Cologne > > >Department of Dermatology > > >]
Krieg, T. [>University of Cologne > > >Department of Dermatology > Center for Biochemistry > >]
Lapiere, C. M. [> > > > > Laboratoire de Biologie des Tissus Conjonctifs > >]
Nusgens, Betty mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs > >]
Aumailley, M. [>University of Cologne > > > > Center for Biochemistry, Center for Molecular Medicine > >]
2006
Cellular and Molecular Life Sciences : CMLS
63
1
82-91
Yes (verified by ORBi)
International
1420-682X
Switzerland
[en] Actins/metabolism ; Cell Adhesion ; Cell Line ; Cell Movement ; Collagen/metabolism ; Extracellular Matrix/metabolism ; Fibroblasts/cytology/enzymology ; Humans ; cdc42 GTP-Binding Protein/genetics/metabolism ; rac1 GTP-Binding Protein/genetics/metabolism ; rhoA GTP-Binding Protein/genetics/metabolism
[en] The GTP-binding proteins RhoA, Cdc42 and Rac1 regulate the organization and turnover of the cytoskeleton and cell-matrix adhesions, structures bridging cells to their support, and translating forces, external or generated within the cell. To investigate the specific requirements of Rho GTPases for biomechanical activities of clonal cell populations, we compared side-by-side stable lines of human fibroblasts expressing constitutively active (CA) RhoA, Cdc42 or Rac1. There was no marked effect of any CA GTPase on cell adhesion to different extracellular matrix proteins. Cell spreading was CA Rho GTPase specific and independent of the extracellular matrix proteins allowing adhesion. Mechanical properties were dramatically restricted by CA RhoA on bi- and in tri-dimensional surroundings, were boosted by CA Rac1 on bi-dimensional surroundings only, and were not or marginally affected by CA Cdc42. In conclusion, the action of Rho GTPases appears to depend on the task cells are performing.
Researchers
http://hdl.handle.net/2268/16167
10.1007/s00018-005-5416-5
The original publication is available at www.springerlink.com

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