ORBi: Danhier Fabienne - Paclitaxel-loaded PEGylated PLGA-based nanoparticles: in vitro and in vivo evaluation

Reference : Paclitaxel-loaded PEGylated PLGA-based nanoparticles: in vitro and in vivo evaluation


	Document type :	Scientific journals : Article
	Discipline(s) :	Engineering, computing & technology : Materials science & engineering Physical, chemical, mathematical & earth Sciences : Chemistry
	To cite this reference:	http://hdl.handle.net/2268/16156

Title :	Paclitaxel-loaded PEGylated PLGA-based nanoparticles: in vitro and in vivo evaluation
Language :	English
Author, co-author :	Danhier, Fabienne [Université catholique de Louvain (UCL) > > Unité de Pharmacie Galénique > >]
	Lecouturier, Nathalie [Université catholique de Louvain (UCL) > > Unité de Pharmacie Galénique > >]
	Vroman, Benoît [Université catholique de Louvain (UCL) > > Unité de Pharmacie Galénique > >]
	Jérôme, Christine [University of Liège (ULg) > Department of Chemistry > Center for Education and Research on Macromolecules (CERM) > >]
	Marchand-Brynaert, Jacqueline [Universté catholique de Louvain (UCL) > > Unité de Chimie Organique et Médicinale > >]
	Feron, Olivier [Université catholique de Louvain (UCL) > > Laboratoire de Pharmacologie et de Thérapeutique > >]
	Préat, Véronique [Université catholique de Louvain (UCL) > > Unité de Pharmacie Galénique > >]
Publication date :	5-Jan-2009
Journal title :	Journal of Controlled Release
Publisher :	Elsevier Science
Volume :	133
Issue/season :	1
Pages :	11-17
Audience :	International
ISSN :	0168-3659
e-ISSN :	1873-4995
City :	Amsterdam
Country :	The Netherlands
Keywords :	[en] biomaterial ; nanomedicine ; organic nanoparticle
Abstract :	[en] The incorporation efficiency of PTX was higher with the nanoprecipitation technique. The release behavior of PTX exhibited a biphasic pattern characterized by an initial burst release followed by a slower and continuous release. The in vitro anti-tumoral activity was assessed using the Human Cervix Carcinoma cells (HeLa) by the MTT test and was compared to the commercial formulation Taxol® and to Cremophor® EL. When exposed to 25 µg/ml of PTX, the cell viability was lower for PTX-loaded nanoparticles than for Taxol® (IC50 5.5 vs 15.5 µg/ml). Flow cytometry studies showed that the cellular uptake of PTX-loaded nanoparticles was concentration and time dependent. Exposure of HeLa cells to Taxol® and PTX-loaded nanoparticles induced the same percentage of apoptotic cells. PTX-loaded nanoparticles showed greater tumor growth inhibition effect in vivo on TLT tumor, compared with Taxol®. Therefore, PTX-loaded nanoparticles may be considered as an effective anticancer drug delivery system for cancer chemotherapy.
Research units :	Center for Education and Research on Macromolecules (CERM)
Funders :	The "FRSM" ; The "Fonds J. Maisin"
Target :	Researchers
Permalink :	http://hdl.handle.net/2268/16156
DOI :	10.1016/j.jconrel.2008.09.086
Other URL :	http://www.elsevier.com/wps/find/journaldescription.cws_home/502690/description#description
	http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T3D-4TMSNPX-2-C&_cdi=4944&_user=532038&_orig=browse&_coverDate=01%2F05%2F2009&_sk=998669998&view=c&wchp=dGLbVzb-zSkWz&md5=3cb31d3781e1b012287221745472de1b&ie=/sdarticle.pdf
Mentions required by the publisher for OA :	The authors acknowledge Journal of Controlled Release (Elsevier) for allowing them to archive this paper.

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