Article (Scientific journals)
The membrane form of the DNA repair protein Ku interacts at the cell surface with metalloproteinase 9.
Monferran, Sylvie; Paupert, Jenny; Dauvillier, Stephanie et al.
2004In EMBO Journal, 23 (19), p. 3758-68
Peer Reviewed verified by ORBi
 

Files


Full Text
3758.full.pdf
Publisher postprint (341.17 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Cell Membrane/metabolism; Cell Movement; DNA Repair; DNA-Binding Proteins/genetics/metabolism; Extracellular Matrix/metabolism; Fluorescent Antibody Technique; Hematopoietic Stem Cells/metabolism; Immunoprecipitation; Leukemia/metabolism/pathology; Matrix Metalloproteinase 9/metabolism; Neoplasm Invasiveness; Tumor Cells, Cultured
Abstract :
[en] The Ku heterodimer (Ku70/Ku80) plays a central role in DNA double-strand breaks repair. Ku is also expressed on the cell surface of different types of cells where its function remains poorly understood. From a yeast two-hybrid screen, we have identified a specific interaction between the core region of Ku80 and the hemopexin domain of metalloproteinase 9 (MMP-9), a key enzyme involved in the degradation of extracellular matrix (ECM) components. Ku associates with MMP-9 on the surface of leukemic cells as demonstrated by co-immunoprecipitation experiments in membrane extracts and double-label immunofluorescence studies. In normal and tumoral migratory cells, Ku80 and MMP-9 colocalize at the periphery of leading edge of cells and cellular invasion of collagen IV matrices was blocked by antibodies directed against Ku70 or Ku80 subunits as well as by Ku80-specific antisense oligonucleotides. Our results indicate that Ku and MMP-9 interact at the cell membrane of highly invasive hematopoietic cells of normal and tumoral origin and document the unexpected importance of the membrane-associated form of Ku in the regulation of ECM remodelling.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Monferran, Sylvie 
Paupert, Jenny  ;  Université Toulouse-Paul Sabatier > Institut de pharmacologie et de biologie structurale
Dauvillier, Stephanie
Salles, Bernard
Muller, Catherine
 These authors have contributed equally to this work.
Language :
English
Title :
The membrane form of the DNA repair protein Ku interacts at the cell surface with metalloproteinase 9.
Publication date :
2004
Journal title :
EMBO Journal
ISSN :
0261-4189
eISSN :
1460-2075
Publisher :
Wiley-Blackwell, United States
Volume :
23
Issue :
19
Pages :
3758-68
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 17 January 2014

Statistics


Number of views
38 (0 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
91
Scopus citations®
without self-citations
80
OpenCitations
 
88

Bibliography


Similar publications



Contact ORBi