Reference : Characterization of the C-Terminal Propeptide Involved in Bacterial Wall Spanning of Alp...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/16076
Characterization of the C-Terminal Propeptide Involved in Bacterial Wall Spanning of Alpha-Amylase from the Psychrophile Alteromonas Haloplanctis
English
Feller, Georges mailto [Université de Liège - ULg > Département des sciences de la vie > Labo de biochimie >]
D'Amico, Salvino [Université de Liège - ULg > Département des sciences de la vie > Labo de Biochimie > >]
Benotmane, A. M. [> > > >]
Joly, F. [> > > >]
Van Beeumen, J. [> > > >]
Gerday, Charles mailto [Université de Liège - ULg > Département des sciences de la vie > Labo de Biochimie > >]
11-May-1998
Journal of Biological Chemistry
273
20
12109-15
Yes (verified by ORBi)
International
0021-9258
[en] The antarctic psychrophile Alteromonas haloplanctis secretes a Ca2+- and Cl--dependent alpha-amylase. The nucleotide sequence of the amy gene and the amino acid sequences of the gene products indicate that the alpha-amylase precursor is a preproenzyme composed by the signal peptide (24 residues), the mature alpha-amylase (453 residues, 49 kDa), and a long C-terminal propeptide or secretion helper (192 residues, 21 kDa). In cultures of the wild-type strain, the 70-kDa precursor is secreted at the mid-exponential phase and is cleaved by a nonspecific protease into the mature enzyme and the propeptide. The purified C-terminal propeptide displays several features common to beta-pleated transmembrane proteins. It has no intramolecular chaperone function because active alpha-amylase is expressed by Escherichia coli in the absence of the propeptide coding region. In E. coli, the 70-kDa precursor is directed toward the supernatant. When the alpha-amylase coding region is excised from the gene, the secretion helper can still promote its own membrane spanning. It can also accept a foreign passenger, as shown by the extracellular routing of a beta-lactamase-propeptide fusion protein.
http://hdl.handle.net/2268/16076

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