[en] BACKGROUND: The effects of proanthocyanidins (PACs), isolated from blackcurrant (Ribes nigrum L.) leaves, on neutrophil accumulation during inflammatory processes were investigated in vivo and in vitro. METHODS: In vivo studies were performed using carrageenin-induced pleurisy in rats pre-treated with PACs. Exudate volume and PMNs accumulation were measured. Leukocyte cell adhesion molecules (LFA-1, Mac-1 and VLA-4) mobilization in circulating granulocytes were analysed by flow cytometry and endothelial cell adhesion molecules (ICAM-1 and VCAM-1) were detected by immunohistochemistry on lung sections. In vitro studies were conducted on endothelial LT2 cells, stimulated with TNF-alpha, to evaluate ICAM-1, IL-8 and VEGF mRNA expression upon PACs treatment. Data sets were examined by one-way analysis of variance (ANOVA) followed by a Scheffe post-hoc test. RESULTS: Pretreatment of the animals with PACs (10, 30 and 60 mg/kg) inhibited dose-dependently carrageenin-induced pleurisy in rats by reducing pleural exudate formation and PMNs infliltration. Leukocyte cell adhesion molecules mobilization was not down-regulated on granulocytes by PACs. Immunohistochemistry on lung sections showed a decreased production of endothelial cell adhesion molecules. In vitro experiments demonstrated that PACs were able to significantly inhibit ICAM-1 but not IL-8 and VEGF165 mRNA expression. Moreover, VEGF121 mRNA expression was dose-dependently enhanced. CONCLUSION: This study provides evidence to support the anti-inflammatory activity of proanthocyanidins is related to an inhibition of leukocyte infiltration which can be explained at least in part by a down-regulation of endothelial adhesion molecules, ICAM-1 and VCAM-1 and that these compounds are capable of modulating TNF-alpha-induced VEGF transcription.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Garbacki, Nancy ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Protéines et glycoprot. de matr.extracell. et membran.basal.
Kinet, Marie
Nusgens, Betty ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Protéines et glycoprot. de matr.extracell. et membran.basal.
Desmecht, Daniel ; Université de Liège - ULiège > Département de morphologie et pathologie > Pathologie spéciale et autopsies
Damas, Jacques ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Language :
English
Title :
Proanthocyanidins, from Ribes Nigrum Leaves, Reduce Endothelial Adhesion Molecules Icam-1 and Vcam-1
Carlos TM, Harlan JM: Leukocyte-endothelial adhesion molecules. Blood 1994, 84:2068-2101.
Panés J, Perry M, Granger DN: Leukocyte-endothelial cell adhesion: avenues for therapeutic intervention. Br J Pharmacol 1999, 126:537-550.
Panés J, Granger DN: Leukocyte-endothelial cell interactions: molecular mechanisms and implications in gastro-intestinal disease. Gastroenterology 1998, 114:1066-1090.
Elices MJ, Osborn L, Takada Y, Crouse C, Luhowskyj S, Hemler M, Lobb RR: VCAM-1 on activated endothelium interacts with the leukocyte integrin VLA-4 at a site distinct from the VLA-4/fibronectin binding site. Cell 1990, 60:577-584.
Neufeld G, Cohen T, Gengrinovitch S, Poltorak Z: Vascular endothelial growth factor (VEGF) and its receptors. FASEB J 1999, 13:9-22.
Baggiolini M, Dewald B, Moser B: Interleukin-8 and related chemotactic cytokines-CXC CC chemokines. Adv Immunol 1994, 55:97-119.
Burns AR, Smith CW, Walker DC: Unique structural features that influence neutrophil emigration into the lung. Physiol Rev 2003, 83:309-336.
Hood JD, Meininger CJ, Ziche M, Granger HJ: VEGF upregulates ecNOS message, protein, and NO production in human endothelial cells. Am J Physiol 1998, 274:H1054-H1058.
Gaudry M, Bregerie O, Andrieu V, El Benna J, Pocidalo MA, Hakim J: Intracellular pool of vascular endothelial growth factor in human neutrophils. Blood 1997, 90:4153-4161.
Taichman NS, Young S, Cruchley AT, Taylor P, Paleolog E: Human neutrophils secrete vascular endothelial growth factor. J Leukoc Biol 1997, 62:397-400.
Seo SM, McIntire LV, Smith CW: Effects of IL-8, Gro-alpha, and LTB4 on the adhesive kinetics of LFA-1 and Mac-1 on human neutrophils. Am J Physiol Cell Physiol 2001, 281:C1568-1578.
Tits M, Angenot L, Poukens P, Warin R, Dierckxsens Y: Prodelphinidins from Ribes nigrum. Phytochemistry 1992, 31:971-973.
Tits M, Poukens P, Angenot L, Diercksens Y: Thin-layer chromatographic analysis of proanthocyanidins from Ribes nigrum leaves. J Pharm Biomed Anal 1992, 10:1097-1100.
Tits M, Angenot L, Damas J, Dierckxsens Y, Poukens P: Anti-inflammatory prodelphinidins from black currant (Ribes nigrum) leaves. Planta Med 1991, 57(suppl 2):A134.
Garbacki N, Angenot L, Bassleer C, Damas J, Tits M: Effects of prodelphinidins isolated from Ribes nigrum on chondrocytes metabolism and COX activity. Naunyn-Schmiedeberg's Arch Pharmacol 2002, 365:434-441.
Garbacki N, Tits M, Damas J: Anti-inflammatory effect of natural proanthocyanidins : pharmacological evaluation on in vivo models. Pflügers Archiv Eur J Physiol 2003, 446:R5.
Garbacki N, Damas J: Some effects of proanthocyanidins isolated from Ribes nigrum on the cardiovascular system of the rat. Fund Clin Pharmacol 2004, 18:270.
Garbacki N, Tits M, Angenot L, Damas J: Inhibitory effects of proanthocyanidins from Ribes nigrum leaves on carrageenin acute inflammatory reactions induced in rats. BMC Pharmacol 2004, 4:25.
Mosmann T: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Meth 1983, 65:55-63.
Deroanne CF, Bonjean K, Servotte S, Devy L, Colige A, Clausse N, Blacher S, Verdin E, Foidart JM, Nusgens BV, Castronovo V: Histone deacetylases inhibitors as anti-angiogenic agents altering vascular endothelial growth factor signaling. Oncogene 2002, 21:427-436.
Kalfin R, Righi A, Del Rosso A, Bagchi D, Generini S, Matucci Cerinic M, Das DK: Activin, a grape seed-derived proanthocyanidin extract, reduces plasma levels of oxidative stress and adhesion molecules (ICAM-1, VCAM-1, and E-selectin) in systemic sclerosis. Free Rad Res 2002, 36:819-825.
Bito T, Roy S, Sen CK, Packer L: Pine bark extract pycnogenol downregulates IFN-γ-induced adhesion of T cells to human keratinocytes by inhibiting inducible ICAM-1 expression. Free Rad Biol Med 2000, 28:219-227.
Bagchi D, Sen CK, Ray SD, Das DK, Bagchi M, Preuss HG, Vinson JA: Molecular mechanisms of cardioprotection by a novel grape seed proanthocyanidin extract. Mut Res 2003, 523-524:87-97.
Houck KA, Ferrara N, Winer J, Cachianes G, Li B, Leung DW: The vascular endothelial growth factor family-identification of a fourth molecular species and characterization of alternative splicing of RNA. Mol Endocrinol 1991, 5:1806-1814.
Cohen T, Nahari D, Cerem-Weiss L, Neufeld G, Levi B: Interleukin-6 induces the expression of vascular endothelial growth factor. J Biol Chem 1996, 271:736-741.
Li J, Perrella MA, Tsai JC, Yet SF, Hsieh CM, Yoshizumi M, Patterson C, Endege WO, Zhou F, Lee ME: Induction of vascular endothelial growth factor gene expression by interleukin-1 beta in rat aortic smooth muscle cells. J Biol Chem 1995, 270:308-312.
Ryuto M, Ono M, Izumi H, Yoshida S, Weich HA, Kohno K, Kuwano M: Induction of vascular endothelial growth factor by tumor necrosis facto alpha in human glioma cells - possible role of SP-1. J Biol Chem 1996, 271:28220-28228.
Khanna S, Roy S, Bagchi D, Bagchi M, Sen CK: Upregulation of oxidant-induced VEGF expression in cultured keratinocytes by a grape seed proanthocyanidin extract. Free Rad Biol Med 2001, 31:38-42.
Khanna S, Venojarvi M, Roy S, Sharma N, Trikha P, Bagchi D, Bagchi M, Sen CK: Dermal wound healing properties of redox-active grape seed proanthocyanidins. Free Rad Biol Med 2002, 33:1089-1096.
Maffei Facino R, Carini M, Aldini G, Berti F, Rossoni G, Bombardelli E, Morazzoni P: Procyanidins from Vitis vinifera seeds protect rabbit heart from ischemia/reperfusion injury: antioxidant intervention and/or iron and copper sequestering ability. Planta Med 1996, 62:495-502.
Aucamp J, Gaspar A, Hara Y, Apostolides Z: Inhibition of xanthine oxidase by catechins from tea (Camellia sinensis). Anticancer Res 1997, 17:4381-4385.
Bagchi D, Garg A, Krohn RL, Bagchi M, Bagchi DJ, Balmoori J, Stohs SJ: Protective effects of grape seed proanthocyanidins and selected antioxidants against TPA-induced hepatic and brain lipid peroxidation and DNA fragmentation and peritoneal macrophage activation in mice. Gen Pharmacol 1998, 30:771-776.
Zhao J, Wang J, Chen Y, Agarwal R: Anti-tumor-promoting activity of a polyphenolic fraction isolated from grape seeds in the mouse skin two-stage initiation-promotion protocol and identification of procyanidin B5-3'-gallate as the most effective antioxidant constituent. Carcinogenesis 1999, 20:1737-1745.
Bagchi D, Bagchi M, Stohs SD, Ray SD, Sen CK, Preuss HG: Cellular protection with proanthocyanidins derived from grape seeds. Ann NY Acad Sci 2002, 957:260-270.