[en] The purpose of the research was to develop and validate a rapid quantification method able to screen many samples of yam bean seeds to determine the content of two toxic polyphenols, namely pachyrrhizine and rotenone. The analytical procedure described is based on the use of an internal standard (dihydrorotenone) and is divided in three steps: microwave assisted extraction, purification by solid phase extraction and assay by ultra high performance liquid chromatography (UHPLC). Each step was included in the validation protocol and the accuracy profiles methodology was used to fully validate the method. The method was fully validated between 0.25mg and 5mg pachyrrhizin per gram of seeds and between 0.58mg/g and 4mg/g for rotenone. More than one hundred samples from different accessions, locations of growth and harvest dates were screened. Pachyrrhizine concentrations ranged from 3.29mg/g to lower than 0.25mg/g while rotenone concentrations ranged from 3.53mg/g to lower than 0.58mg/g. This screening along with principal component analysis (PCA) and discriminant analysis (DA) analyses allowed the selection of the more interesting genotypes in terms of low concentrations of these two toxic polyphenols.
Research center :
LDRI Centre Interfacultaire de Recherche du Médicament - CIRM
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Lautié, Emmanuelle
Rozet, Eric ; Université de Liège - ULiège > Département de pharmacie > Chimie analytique
Hubert, Philippe ; Université de Liège - ULiège > Département de pharmacie > Chimie analytique
Vandelaer, Nicolas
Billard, François
zum Felde, Thomas
Grünenberg, Wolfgang-J.
Quetin-Leclercq, Joëlle; Université Catholique de Louvain - UCL > LDRI > Laboratoire de Pharmacognosie
Language :
English
Title :
Fast method for the simultaneous quantification of toxic polyphenols applied to the selection of genotypes of yam bean (Pachyrhizus sp.) seeds.
Publication date :
September 2013
Journal title :
Talanta
ISSN :
0039-9140
eISSN :
1873-3573
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
117
Pages :
94-101
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
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