AMP-Activated Protein Kinases/metabolism; Animals; Chickens/metabolism/physiology; Fasting/physiology; Hypothalamus/metabolism; Male; Signal Transduction
Abstract :
[en] An experiment was conducted to investigate the effects of fasting and re-feeding on hypothalamic 5'-AMP-activated protein kinase (AMPK) levels and (an)orexigenic neuropeptides. Male Arbor Acres chicks (7-day-old, n=160) were allocated to four equal treatment groups: control chicks (fed ad libitum for 48 h, C48), chicks that were fasted for 48 h (F48), chicks that were first fasted for 48 h and then re-fed for 24h (F48C24), and chicks that were fed ad libitum for 72h (C72). Fasting for 48 h significantly (P<0.05) increased the ratio of phosphorylated AMPKalpha to total AMPKalpha and phosphorylated LKB1 to total LKB1, whereas re-feeding for 24h reduced these ratios to that of the ad libitum fed C72 chicks. The gene expressions of agouti-related peptide (AgRP), neuropeptide Y (NPY), melanocortin receptor 4, melanin-concentrating hormone, prepro-orexins and carnitine palmitoyltransferase-1 were significantly (P<0.05) increased in the fasted chicks relative to the ad libitum fed C48 group. The gene expression of pro-opiomelanocortin (POMC), as well as cocaine- and amphetamine-regulated transcript (CART) was not affected by the nutritional status. Fasting significantly (P<0.05) decreased the mRNA levels of fatty acid synthase (FAS) and sterol regulatory element binding protein-1 (SREBP-1). The results suggest that the LKB1/AMPK signal pathway is involved in the energy homeostasis of fasted chicks, and its possible role in feed intake regulation might be mediated by the AgRP/NPY rather than the POMC/CART pathway.
Disciplines :
Animal production & animal husbandry
Author, co-author :
Song, Zhigang
Liu, Lei
Yue, Yunshuang
Jiao, Hongchao
Lin, Hai
Sheikhahmadi, Ardashir
Everaert, Nadia ; KU Leuven > Department of Biosystems > Division Livestock-Nutrition-Quality
Decuypere, Eddy
Buyse, Johan
Language :
English
Title :
Fasting alters protein expression of AMP-activated protein kinase in the hypothalamus of broiler chicks (Gallus gallus domesticus).
Publication date :
2012
Journal title :
General and Comparative Endocrinology
ISSN :
0016-6480
eISSN :
1095-6840
Publisher :
Elsevier, United States - New York
Volume :
178
Issue :
3
Pages :
546-55
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2012 Elsevier Inc. All rights reserved.
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