Article (Scientific journals)
Expression and Induction of Drug-Metabolizing Enzymes in Cultured Fetal Rat Hepatocytes
Kremers, Pierre; Roelandt, L.; Stouvenakers, Nadine et al.
1994In Cell Biology and Toxicology, 10 (2), p. 117-25
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Abstract :
[en] An in vitro experimental model, fetal rat hepatocytes in culture, was metabolically characterized. Several enzymatic activities were expressed in these hepatocytes, namely, testosterone hydroxylations. Hepatocytes cultured up to 3 weeks in the presence of dexamethasone and phenobarbital still expressed some drug-metabolizing enzyme activities (e.g., ECOD). The enzymatic activities were measured both directly on monolayers during culture and on the corresponding harvested and homogenized cells. The results correlate perfectly with each other. The 'on cell' procedure allows us to repeat the assay or to measure several activities on the same cells at different time intervals. The presence of dexamethasone in the culture medium allows the expression and the induction of several cytochrome P450 isoenzymes, namely, those hydroxylating testosterone. This makes the model particularly attractive for induction experiments as well as for metabolic or toxicological studies needing longer treatments.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Kremers, Pierre 
Roelandt, L.
Stouvenakers, Nadine 
Goffinet, Gerhard 
Thomé, Jean-Pierre ;  Université de Liège - ULiège > Département Biologie, Ecologie et Evolution > Ecologie animale et écotoxicologie
Language :
English
Title :
Expression and Induction of Drug-Metabolizing Enzymes in Cultured Fetal Rat Hepatocytes
Publication date :
April 1994
Journal title :
Cell Biology and Toxicology
ISSN :
0742-2091
eISSN :
1573-6822
Publisher :
Kluwer Academic Publishers, Netherlands
Volume :
10
Issue :
2
Pages :
117-25
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
grant of the national impulse program 'Health Hazards' of the Bdgian Ministry of Science and Technology (contract HH46)
FRSM - Fonds de la Recherche Scientifique Médicale [BE]
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since 26 November 2013

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