[en] This study examines the production, characterization and bioactivity on plant cell cultured in vitro of exopolysaccharides (EPS) from Syncephalastrum racemosum CBS 443.59. Firstly, the influence of the fungus culture condition in shake flasks (pH, temperature and different carbon and nitrogen sources) on EPS and biomass production were evaluated. In order to enhance EPS production, a new protocol based on two-stage pH fermentation in a 3 L stirred fermentor was developed. Under this condition, EPS production increased by 3.55 times, compared to a constant pH process, reaching a maximal EPS concentration of 2.62 g/L. Structurally, the EPS contains a polyglucuronic acid backbone, linked essentially with mannose and fucose units and some galactose and glucose units. The bioactivity of EPS as inducer of defence reactions in plant suspension-cultured cells was also studied. Our results show, for first time, that EPS from S. racemosum CBS 443.59 induces, depending on the concentration, PAL activation and H2O2 synthesis in Arabidospsis thaliana cell suspensions.
Disciplines :
Chemistry
Author, co-author :
Valepyn, Emmanuel ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Cabrera, Juan-Carlos; MateriaNova, 1 rue des foudriers, 7822 Ghislenghien
Richel, Aurore ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Paquot, Michel ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Language :
English
Title :
Water soluble exo-polysaccharide from Syncephalastrum racemosum, a strong inducer of plant defence reactions
Publication date :
January 2014
Journal title :
Carbohydrate Polymers
ISSN :
0144-8617
eISSN :
1879-1344
Publisher :
Elsevier Science, Philadelphia, United States
Volume :
101
Pages :
941-946
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
BIOTISS
Funders :
FEDER - Fonds Européen de Développement Régional [BE]
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