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Second harmonic generation from tyrosine containing peptides
Nasir, Mehmet Nail; Bergmann, Emeric; Benichou, Emmanuel et al.
2013In Proceedings of SPIE: The International Society for Optical Engineering, 8817
Peer reviewed
 

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Keywords :
iturin; surfactin; mycosubtilin; bacillomycin; Lipopeptide; second harmonic generation; non linear optics; antimicrobial; hemolytic; membrane
Abstract :
[en] The Second Harmonic Generation (SHG) response from Tyrosine-containing peptides at the air-water interface is presented. First, the quadratic hyperpolarizability of the aromatic amino acid Tyrosine obtained by Hyper Rayleigh Scattering is reported, demonstrating its potentiality as an endogenous molecular probe for SHG studies. Then, the single Tyrosine antimicrobial peptide Mycosubtilin is monitored at the air-water interface and compared to another peptide, Surfactin, lacking a Tyrosine residue. Adsorption kinetics and polarization analysis of the SHG intensity for the peptide monolayers clearly demonstrate that the SHG response from Mycosubtilin arises from Tyrosine. Besides, it confirms that indeed Tyrosine can be targeted as an endogenous molecular probe.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Nasir, Mehmet Nail ;  Université de Liège - ULiège > Chimie et bio-industries > Chimie générale et organique
Bergmann, Emeric
Benichou, Emmanuel
Russier-Antoine, Isabelle
Lascoux, Noëlle
Jonin, Christian
Besson, Françoise
Brevet, Pierre-François
Language :
English
Title :
Second harmonic generation from tyrosine containing peptides
Publication date :
2013
Event name :
Nanobiosystems: Processing, Characterization, and Applications VI
Event place :
San Diego, United States - California
Event date :
Août 2013
By request :
Yes
Audience :
International
Journal title :
Proceedings of SPIE: The International Society for Optical Engineering
ISSN :
0277-786X
eISSN :
1996-756X
Publisher :
International Society for Optical Engineering, Bellingham, United States - Washington
Volume :
8817
Peer reviewed :
Peer reviewed
Commentary :
Conférence donnée par Pr. P-F. Brevet.
Available on ORBi :
since 29 October 2013

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