Article (Scientific journals)
Moritella cold-active dihydrofolate reductase: Are there natural limits to optimization of catalytic efficiency at low temperature?
Xu, Y.; Feller, Georges; Gerday, Charles et al.
2003In Journal of Bacteriology, 185 (18), p. 5519-5526
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Abstract :
[en] Adapting metabolic enzymes of microorganisms to low temperature environments may require a difficult compromise between velocity and affinity. We have investigated catalytic efficiency in a key metabolic enzyme (dihydrofolate reductase) of Moritella profunda sp. nov., a strictly psychrophilic bacterium with a maximal growth rate at 2degreesC or less. The enzyme is monomeric (M-r = 18,291), 55% identical to its Escherichia coli counterpart, and displays T-m and denaturation enthalpy changes much lower than E. coli and Thermotoga maritima homologues. Its stability curve indicates a maximum stability above the temperature range of the organism, and predicts cold denaturation below 0degreesC. At mesophilic temperatures the apparent K-m value for dihydrofolate is 50- to 80-fold higher than for E. coli, Lactobacillus casei, and T. maritima dihydrofolate reductases, whereas the apparent K-m value for NADPH, though higher, remains in the same order of magnitude. At 5degreesC these values are not significantly modified. The enzyme is also much less sensitive than its E. coli counterpart to the inhibitors methotrexate and trimethoprim. The catalytic efficiency (k(cat)/K-m) with respect to dihydrofolate is thus much lower than in the other three bacteria. The higher affinity for NADPH could have been maintained by selection since NADPH assists the release of the product tetrahydrofolate. Dihydrofolate reductase adaptation to low temperature thus appears to have entailed a pronounced trade-off between affinity and catalytic velocity. The kinetic features of this psychrophilic protein suggest that enzyme adaptation to low temperature may be constrained by natural limits to optimization of catalytic efficiency.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Xu, Y.
Feller, Georges ;  Université de Liège - ULiège > Département des sciences de la vie > Labo de biochimie
Gerday, Charles ;  Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)
Glansdorff, N.
Language :
English
Title :
Moritella cold-active dihydrofolate reductase: Are there natural limits to optimization of catalytic efficiency at low temperature?
Publication date :
September 2003
Journal title :
Journal of Bacteriology
ISSN :
0021-9193
eISSN :
1098-5530
Publisher :
Amer Soc Microbiology, Washington, United States - Washington
Volume :
185
Issue :
18
Pages :
5519-5526
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 26 January 2010

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