Article (Scientific journals)
Obesity phenotype is related to NLRP3 inflammasome activity and immunological profile of visceral adipose tissue
ESSER, Nathalie; L'Homme, Laurent; DE ROOVER, Arnaud et al.
2013In Diabetologia, 56, p. 2487-2497
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Keywords :
Adipose tissue .; Inflammatory cytokines; Macrophages .; Metabolic disorders .; NLRP3 inflammasome .; Obesity
Abstract :
[en] Aims/hypothesis Obesity is a heterogeneous condition comprising both individuals who remain metabolically healthy (MHO) and those who develop metabolic disorders (metabolically unhealthy, MUO). Adipose tissue is also heterogeneous in that its visceral component is more frequently associated with metabolic dysfunction than its subcutaneous component. The development of metabolic disorders is partly mediated by the NLR family pyrin domain containing-3 (NLRP3) inflammasome, which increases the secretion of inflammatory cytokines via activation of caspase-1. We compared the immunological profile and NLRP3 activity in adipose tissue between MUO and MHO individuals. Methods MHO and MUO phenotypes were defined, respectively, as the absence and the presence of the metabolic syndrome. Cellular composition and intrinsic inflammasome activity were investigated by flow cytometry, quantitative RTPCR and tissue culture studies in subcutaneous and visceral adipose tissue from 23 MUO, 21 MHO and nine lean individuals. Results We found significant differences between the three study groups, including an increased secretion of IL-1β, increased expression of IL1B and NLRP3, increased number of adipose tissue macrophages and decreased number of regulatory T cells in the visceral adipose tissue of MUO patients compared with MHO and lean participants. In macrophages derived from visceral adipose tissue, both caspase-1 activity and IL-1β levels were higher in MUO patients than in MHO patients. Furthermore, caspase-1 activity was higher in CD11c+CD206+ adipose tissue macrophages than in CD11c−CD206+ cells. Conclusions/interpretation The MUO phenotype seems to be associated with an increased activation of the NLPR3 inflammasome in macrophages infiltrating visceral adipose tissue, and a less favourable inflammatory profile compared with the MHO phenotype.
Disciplines :
Endocrinology, metabolism & nutrition
Immunology & infectious disease
Author, co-author :
ESSER, Nathalie  ;  Centre Hospitalier Universitaire de Liège - CHU > Frais communs médecine
L'Homme, Laurent ;  Université de Liège - ULiège > GIGA-R : Virologie - Immunologie
DE ROOVER, Arnaud ;  Centre Hospitalier Universitaire de Liège - CHU > Chirurgie abdominale- endocrinienne et de transplantation
KOHNEN, Laurent ;  Centre Hospitalier Universitaire de Liège - CHU > Chirurgie abdominale- endocrinienne et de transplantation
SCHEEN, André  ;  Centre Hospitalier Universitaire de Liège - CHU > Diabétologie,nutrition, maladies métaboliques
MOUTSCHEN, Michel  ;  Centre Hospitalier Universitaire de Liège - CHU > Maladies infectieuses et médecine interne générale
Piette, Jacques ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Virologie - Immunologie
Legrand-Poels, Sylvie ;  Université de Liège - ULiège > GIGA-R : Virologie - Immunologie
PAQUOT, Nicolas ;  Centre Hospitalier Universitaire de Liège - CHU > Diabétologie,nutrition, maladies métaboliques
Language :
English
Title :
Obesity phenotype is related to NLRP3 inflammasome activity and immunological profile of visceral adipose tissue
Publication date :
November 2013
Journal title :
Diabetologia
ISSN :
0012-186X
eISSN :
1432-0428
Publisher :
Springer Verlag, Berlin, Germany
Volume :
56
Pages :
2487-2497
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 14 October 2013

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