Article (Scientific journals)
A perspective on cold enzymes: Current knowledge and frequently asked questions
Marx, J. C.; Blaise, Vinciane; Collins, T. et al.
2004In Cellular and Molecular Biology, 50 (5), p. 643-655
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Keywords :
cold-adaptation; psychrophilic enzymes; catalytic cycle; kinetic constants; flexibility; stability; cold-activity
Abstract :
[en] Studies on psychrophilic enzymes to determine the structural features important for cold-activity have attracted increased attention in the last few years. This enhanced interest is due to the attractive properties of such proteins, i.e. a high specific activity and a low thermal stability, and thus, these enzymes constitute a tremendous potential for fundamental research and biotechnological applications. This review examines the impact of low temperatures on life, the diversity of adaptation to counteract these effects and gives an overview of the features proposed to account for low thermal stability and cold-activity, following the chronological order of the catalytic cycle phases. Moreover, we present an overview of recent techniques used in the analysis of the flexibility of a protein structure which is an important concept in cold-adaptation; an overview of biotechnological potential of psychrophilic enzymes and finally, a few frequently asked questions about cold-adaptation and their possible answers.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Marx, J. C.
Blaise, Vinciane ;  Université de Liège - ULiège > Département des sciences et gestion de l'environnement > Département des sciences et gestion de l'environnement
Collins, T.
D'Amico, Salvino ;  Université de Liège - ULiège > GIGA-Research
Delille, D.
Gratia, E.
Hoyoux, A.
Huston, A. L.
Sonan, G.
Feller, Georges ;  Université de Liège - ULiège > Département des sciences de la vie > Labo de biochimie
Gerday, Charles ;  Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)
Language :
English
Title :
A perspective on cold enzymes: Current knowledge and frequently asked questions
Publication date :
July 2004
Journal title :
Cellular and Molecular Biology
ISSN :
0145-5680
eISSN :
1165-158X
Publisher :
C.M.B. Association, France
Volume :
50
Issue :
5
Pages :
643-655
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 26 January 2010

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