Protective effect of a new biomaterial against the development of experimental osteoarthritis lesions in rabbit: a pilot study evaluating the intra-articular injection of alginate-chitosan beads dispersed in an hydrogel.
[en] Objective: This study aimed to evaluate the structural benefit of a new biomaterial composed
of alginate-chitosan (AC) beads dispersed in an hydrogel (H) derived from chitosan on the
development of osteoarthritis (OA) in rabbit.
Design: OA was induced by the surgical transection of the anterior cruciate ligament in
rabbits. Animals received a single intra-articular injection (900 μl) of AC beads in H
hydrogel, H hydrogel alone or saline one week after surgery. OA development was followed
by X-rays. Blood samples were collected throughout the study to measure biological markers
(PGE2 and CRP). Macroscopic observation and histological evaluation of articular cartilage
and synovial membrane were performed 6 weeks after surgery.
Results: AC beads in H hydrogel prevented from the development of OA based on the
reduction of the Kellgren & Lawrence (K&L) score. It also significantly reduced the
histological score of cartilage lesion severity. This effect was homogenous on every joint
compartment. It was due to a significant effect on cartilage structure and cellularity scores.
The injection of AC beads in H hydrogel also tended to reduce the synovial membrane
inflammation. No significant variation of biological markers was noted.
Conclusions: The present pilot study provides interesting and promising results for the use of
AC beads in H hydrogel in animal. It indeed prevented the development of OA cartilage
lesions without inflammatory signs. The potencies of this biomaterial to protect OA joint
should be further documented. It could then represent a new alternative for
viscosupplementation in human OA management.
Disciplines :
Rheumatology
Author, co-author :
Oprenyeszk, Frédéric ; Université de Liège - ULiège > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.)
Chausson, Mickael
Maquet, Véronique
Dubuc, Jean-Emile
Henrotin, Yves ; Université de Liège - ULiège > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.)
Language :
English
Title :
Protective effect of a new biomaterial against the development of experimental osteoarthritis lesions in rabbit: a pilot study evaluating the intra-articular injection of alginate-chitosan beads dispersed in an hydrogel.
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