[en] Glucosinolates, natural compounds found in Brassicaceae, can easily be transformed into desulfo-glucosinolates by action of Helix pomatia sulfatase. The recombinant beta -O-glucosidase from Caldocellum saccharolyticum does not catalyse glucosinolate degradation but can hydrolyse desulfo-glucosinolates (thio-D-glucosidic substrates) to produce the corresponding pure nitriles, including valuable homochiral representatives.
Ettlinger M., Lundeen A. (1956) The structure of sinigrin and sinalbin; an enzymatic rearrangement. J. Am. Chem. Soc. 78:4172-4173.
Fenwick R.G., Heaney R.K., Mullin W. (1983) Glucosinolates and their breakdown products in food and food plants. CRC Crit. Rev. Food Sci. Nutr. 18:123-201.
Kirk L., Mustakas E., Grifin J., Booth A. (1971) Crambe seed processing. Decomposition of glucosinolates (thioglucosides) with chemical additives. J. Am. Oil Chem. Soc. 48:845-850.
Larsen P. (1981) Glucosinolates. The Biochemistry of Plants. A Comprehensive Treatise , Stumpf P, Conn E, eds. New York: Academic Press; 7:501-525.
Leoni O., Iori R., Haddoum T., Marlier M., Wathelet J.-P., Rollin P., Palmieri S. (1998) Approach to the use of immobilised sulfatase for analytical purposes and for the production of desulfo-glucosinolates. Ind. Crops Prod. 7:335-343.
Palmieri S., Iori R., Joseph B., Rollin P. (1993) Myrosinase and hydrolytic degradation of natural and synthetic glucosinolates. Colloque Glucosinolates '10 Années de Recherche' CETIOM Ardon, France, 13-14 January 1993 57-62.
Plant A., Oliver J., Patchett M., Daniel R., Morgan H. (1988) Stability and substrate specificity of a beta-glucosidase from the thermophilic bacterium Tp8 cloned into Escherichia coli. Arch. Biochem. Biophys. 262:181-188.
Thies W. (1979) Detection and utilisation of a glucosinolate sulfo-hydrolase in the edible snail Helix pomatia. Naturwissenschaften 66:364-365.
Thies W. (1988) Isolation of sinigrin and glucotropaeolin from cruciferous seeds. Fat Sci. Technol. 8:311-314.
Vangheesdaele G., Fournier N. (1977) La dégradation des thioglucosides par les sels de cuivre (application au dosage de la sinigrine dans les graines de moutarde). Ann. Technol. Agric. 26:499-510.
Viaud M.-C., Rollin P., Latxague L., Gardrat C. (1992) Synthethic studies on indole glucosinolates. Part 1. Synthesis of glucobrassicin and its 4- and 5-methoxy derivatives. J. Chem. Res. 207:1669-1681.
Visentin M., Tava A., Iori R., Palmieri S. (1992) Isolation and identification of trans-4-(methylthio)-3-butenyl glucosinolate from radish root (Raphanus sativus). J. Agric. Food Chem. 40:1687-1691.
