Relationships between in vitro lymphoproliferative responses and levels of contaminants in blood of free-ranging adult harbour seals (Phoca vitulina) from the North Sea

Dupont, Aurélie; Siebert, Ursula; Covaci, Adrian; Weijs, Liesbeth; Eppe, Gauthier; Debier, Cathy; De Pauw-Gillet, Marie-Claire; Das, Krishna

doi:10.1016/j.aquatox.2013.08.014

Article (Scientific journals)

Relationships between in vitro lymphoproliferative responses and levels of contaminants in blood of free-ranging adult harbour seals (Phoca vitulina) from the North Sea

Dupont, Aurélie; Siebert, Ursula; Covaci, Adrian et al.

2013 • In Aquatic Toxicology, 142-143, p. 210-220

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/156095

DOI
10.1016/j.aquatox.2013.08.014

PubMed
24051082

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Keywords :

marine mammals; pollutants; in vitro; Phoca vitulina; blood; contaminants; lymphoproliferation; immune parameters; immunotoxicology

Abstract :

[en] tIn vitro culture of peripheral blood leucocytes (PBLs) is currently used in toxicological studies of marinemammals. However, blood cells of wild individuals are exposed in vivo to environmental contaminantsbefore being isolated and exposed to contaminants in vitro. The aim of this study was to highlightpotential relationships between blood contaminant levels and in vitro peripheral blood lymphocyteproliferation in free-ranging adult harbour seals (Phoca vitulina) from the North Sea. Blood samplesof 18 individuals were analyzed for trace elements (Fe, Zn, Se, Cu, Hg, Pb, Cd) and persistent organiccontaminants and metabolites ( PCBs, HO-PCBs, PBDEs, 2-MeO-BDE68 and 6-MeO-BDE47, DDXs,hexachlorobenzene, oxychlordane, trans-nonachlor, pentachlorophenol and tribromoanisole). The samesamples were used to determine the haematology profiles, cell numbers and viability, as well as thein vitro ConA-induced lymphocyte proliferation expressed as a stimulation index (SI). Correlation tests(Bravais-Pearson) and Principal Component Analysis with multiple regression revealed no statisticallysignificant relationship between the lymphocyte SI and the contaminants studied. However, the numberof lymphocytes per millilitre of whole blood appeared to be negatively correlated to pentachlorophenol(r = −0.63, p = 0.005). In adult harbour seals, the interindividual variations of in vitro lymphocyte pro-liferation did not appear to be directly linked to pollutant levels present in the blood, and it is likelythat other factors such as age, life history, or physiological parameters have an influence. In a generalmanner, experiments with in vitro immune cell cultures of wild marine mammals should be designed soas to minimize confounding factors in which case they remain a valuable tool to study pollutant effectsin vitro.

Research center :

MARE - Centre Interfacultaire de Recherches en Océanologie - ULiège

Disciplines :

Aquatic sciences & oceanology
Environmental sciences & ecology

Author, co-author :

Dupont, Aurélie ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Océanologie

Siebert, Ursula

Covaci, Adrian

Weijs, Liesbeth

Eppe, Gauthier ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique

Debier, Cathy

De Pauw-Gillet, Marie-Claire ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie

Das, Krishna ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Océanologie

Language :

English

Title :

Relationships between in vitro lymphoproliferative responses and levels of contaminants in blood of free-ranging adult harbour seals (Phoca vitulina) from the North Sea

Publication date :

2013

Journal title :

Aquatic Toxicology

ISSN :

0166-445X

eISSN :

1879-1514

Publisher :

Elsevier Science, Amsterdam, Netherlands

Volume :

142-143

Pages :

210-220

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://www.sciencedirect.com/science/article/pii/S0166445X13002221#
http://reflexions.ulg.ac.be/Phoques
[en] http://reflexions.ulg.ac.be/en/Seals

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]

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