Reference : A new model for human intestinal preservation: Comparison of University of Wisconsin and...
Scientific journals : Article
Human health sciences : Gastroenterology & hepatology
Human health sciences : Immunology & infectious disease
Human health sciences : Surgery
http://hdl.handle.net/2268/1560
A new model for human intestinal preservation: Comparison of University of Wisconsin and Celsior preservation solutions
English
De Roover, Arnaud mailto [Centre Hospitalier Universitaire de Liège - CHU > > Chirurgie abdominale- endocrinienne et de transplantation >]
de Leval, Laurence mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Gilmaire, Julie [Centre Hospitalier Universitaire de Liège - CHU > > Frais communs Biologie Clinique >]
Detry, Olivier mailto [Centre Hospitalier Universitaire de Liège - CHU > > Chirurgie abdominale- endocrinienne et de transplantation >]
Boniver, Jacques mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques]
Honore, Pierre mailto [Centre Hospitalier Universitaire de Liège - CHU > > Chirurgie abdominale- endocrinienne et de transplantation >]
Meurisse, Michel mailto [Université de Liège - ULg > Département des sciences cliniques > Chirurgicale abdominale]
Mar-2004
Transplantation Proceedings
Elsevier Science Inc
36
2
270-272
Yes (verified by ORBi)
International
0041-1345
New York
[en] organ transplantation ; preservation ; intestinal failure
[en] AIM: We compared University of Wisconsin (UW) and Celsior preservation solutions using a new model of human intestinal preservation that mimics the clinical conditions of small bowel procurement. METHODS: Intestinal grafts were harvested from four multiorgan donors. After classic warm dissection for organ procurement, an ileal segment of 50 cm was immediately flushed with Celsior. After the perfusion of the abdominal organs with UW, a second segment of adjacent ileum was harvested. The two intestinal grafts were then divided into segments by stapling, before immersion into the corresponding preservation solution (Celsior or UW) for 0-, 6-, 12-, or 24-hour incubation at 4 degrees C. A histological score was graded after blinded examination of three random specimens within each ileal graft for each duration of preservation. RESULTS: Control specimens showed normal histology. After 6 hours of preservation, most villi showed complete epithelial detachment although the crypts appeared intact. After 12 hours of preservation, a larger proportion of the villi showed extensive epithelial sloughing. After 24 hours, the damage involved the entire mucosa with the crypt epithelium largely detached from the basal membrane. No statistical difference in histological score was observed between the two preservation solutions. CONCLUSION: This study showed severe histological alterations of graft mucosa after short periods of preservation by UW or Celsior solutions. This model may be useful to evaluate improvements in the quality of preservation of human intestinal transplants.
Professionals ; Students ; General public
http://hdl.handle.net/2268/1560
10.1016/j.transproceed.2003.12.003

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