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A mucin-enriched fermentation model to assess prebiotic potential of new indigestible carbohydrates
Boudry, Christelle; Tran, Thi Hanh Tham; Blaise, Yannick et al.
2013In 64th Annual Meeting of the European Federation of Animal Science
 

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Keywords :
prebiotic; in vitro fermentation; Lactobacillus; real time PCR
Abstract :
[en] Screening the prebiotic potential of novel indigestible carbohydrates (ICH) is a challenge for feed and food industry and in vitro models are increasingly used for such purposes. Recently extracellular binding proteins responsible for the adherence to intestinal mucus were described for several Lactobacillus species. As this genus is known for its beneficial effect on gut health, we enriched the in vitro gas fermentation model with mucin in order to evaluate the prebiotic potential of 5 ICH. Mucin-coated microcosms (MCM) were prepared as described by Van den Abbeele et al. (2012, Microbial Biotechnology, 5, 106-115) and introduced in the fermentation bottles with an inoculum prepared from fresh faeces of 3 sows mixed with a nutritive buffer solution. Fermentation was performed at 39°C, using 200 mg of substrate, 30 ml of inoculum and 6 MCM, yielding approx. 20 mg mucin each, in 140 ml glass bottles. A first study was performed with inulin and cellulose as substrates, with and without mucus in the bottles. A second study was performed with 5 substrates (inulin, IMO, beet pulp POS, cellobiose and gluconate) in presence of mucus. After 8 and 72h, SCFA and the microflora of fermentation broth was analysed as well as the microflora on the MCM. The comparison of the microflora evolution with and without mucus showed a better development of the Lactobacillus in the fermentation broth, mainly in presence of inulin. The development of the Lactobacillus genus allowed the classification of the 5 substrates tested in the second study (Inulin > IMO > Gluconate > Cellobiose > POS)(P < 0.05) which was not possible without mucus (P > 0.05). Inulin and IMO showed also the highest development of Bifidobacteria (P < 0.05) and the highest levels of butyrate production (P < 0.05) compared to the three other substrates, indicating a high prebiotic potential.
Disciplines :
Agriculture & agronomy
Author, co-author :
Boudry, Christelle ;  Université de Liège - ULiège > Sciences agronomiques > Zootechnie
Tran, Thi Hanh Tham ;  Université de Liège - ULiège > Doct. sc. agro. & ingé. biol.
Blaise, Yannick ;  Université de Liège - ULiège > Sciences agronomiques > Zootechnie
Thewis, André ;  Université de Liège - ULiège > Sciences agronomiques > Zootechnie
Bindelle, Jérôme  ;  Université de Liège - ULiège > Sciences agronomiques > Zootechnie
Language :
English
Title :
A mucin-enriched fermentation model to assess prebiotic potential of new indigestible carbohydrates
Publication date :
29 August 2013
Event name :
64th Annual Meeting of the European Federation of Animal Science
Event organizer :
EAAP
Event place :
Nantes, France
Event date :
du 25 au 30 Août 2013
Audience :
International
Main work title :
64th Annual Meeting of the European Federation of Animal Science
Publisher :
Wageningen Academic Publishers, Wageningen, Netherlands
Edition :
First published 2013
ISBN/EAN :
978-90-8686-228-3
Pages :
551
Name of the research project :
HYDRASANTE
Funders :
Service public de Wallonie : Direction générale opérationnelle de l'économie, de l'emploi et de la recherche - DG06
Available on ORBi :
since 03 September 2013

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