Poster (Scientific congresses and symposiums)
Modelization of photosynthetic and respiratory maximal activities as a function of culture parameters in Chlamydomonas reinhardtii using design of experiment and data analysis by JMP (SAS Institute)
Gerin, Stéphanie; Mathy, Grégory; Franck, Fabrice
201316th International Congress on Photosynthesis Research
 

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Keywords :
Bioenergetics; Design of experiment; JMP; Cell culture; Chlamydomonas; Mitochondrion; Chloroplast; Respiration; Photosynthesis
Abstract :
[en] We aimed to modelize the dependence of photosynthetic as well as cytochromial and alternative respiratory maximal activities upon different culture parameters, i.e. light intensity and acetate, carbon dioxide, nitrate and ammonium concentrations, in the model unicellular green alga Chlamydomonas reinhardtii. Culture parameters were submitted to a dedicated statistical software (JMP 10.0, SAS Institute) for the generation of a design of experiment. Measurements of oxygen concentrations were carried out to quantify maximal activities. JMP was further used to detect culture parameters exerting a statistically significant effect on maximal activities and to modelize the dependence of maximal activities upon these culture parameters of interest.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Gerin, Stéphanie ;  Université de Liège - ULiège > Labo de Bioénergétique
Mathy, Grégory;  UCB Pharma > Cell Culture Process Sciences
Franck, Fabrice ;  Université de Liège - ULiège > Labo de Bioénergétique
Language :
English
Title :
Modelization of photosynthetic and respiratory maximal activities as a function of culture parameters in Chlamydomonas reinhardtii using design of experiment and data analysis by JMP (SAS Institute)
Publication date :
August 2013
Number of pages :
A0
Event name :
16th International Congress on Photosynthesis Research
Event place :
Saint-Louis, United States - Missouri
Event date :
du 11 août 2013 au 16 août 2013
Audience :
International
Name of the research project :
Study of metabolic plasticity in response to disruptions of chloroplastic function in Chlamydomonas reinhardtii
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
ULg - Université de Liège [BE]
Funding text :
Université de Liège - Missions scientifiques du Patrimoine
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since 23 August 2013

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