Poster (Scientific congresses and symposiums)
Research of New Enzyme Producing Strains in the Gut of the Termite Reticulitermes santonensis
Tarayre, Cédric; Bauwens, Julien; Mattéotti, Christel et al.
2013COSM'ING 2013
 

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Abstract :
[en] Termites contain a complex microflora inside of their guts. Inferior termites contain bacteria, mycetes and protists that interact to degrade vegetable components. These strains act as consortia to break natural materials by secreting various enzymes. Our aim was the isolation and cultivation of microorganisms in order to produce new enzymes that can be further used in green chemistry. Termites were fed with different diets: pinewood, microcristalline cellulose (added with lignin or not), α-cellulose (added with lignin or not) and birchwood xylan. Then, dissections were realized to isolate interesting strains. All the microorganisms were subjected to enzyme assays. That technique allowed us to isolate and to cultivate various strains of bacteria, molds and protists. Three strains of bacteria, two strains of molds and one strain of protist were isolated and displayed different enzymatic activities. The bacteria Bacillus subtilis strain ABGx, Bacillus sp. strain CTGx and Chryseobacterium sp. strain CTGx displayed amylase, cellulase and xylanase activities. The molds Trichoderma virens strain CTGx and Sarocladium kiliense strain CTGx were also able to produce those enzymes. However, the protist Poterioochromonas sp. was found to produce only amylase. In conlusion, the termite gut is a complex culivation medium that provides a habitat for many microorganisms that show interesting enzymatic activities.
Disciplines :
Microbiology
Author, co-author :
Tarayre, Cédric ;  Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
Bauwens, Julien ;  Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Mattéotti, Christel
Brasseur, Catherine ;  Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Millet, Catherine ;  Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique
Guiot, Pierre
Vandenbol, Micheline ;  Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique
Portetelle, Daniel ;  Université de Liège - ULiège > Chimie et bio-industries > Microbiologie et génomique
De Pauw, Edwin  ;  Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)
Haubruge, Eric  ;  Université de Liège - ULiège > Vice-Recteur de Gembloux Agro Bio Tech
Francis, Frédéric  ;  Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive
Thonart, Philippe ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Destain, Jacqueline ;  Université de Liège - ULiège > Chimie et bio-industries > Bio-industries
More authors (3 more) Less
Language :
English
Title :
Research of New Enzyme Producing Strains in the Gut of the Termite Reticulitermes santonensis
Publication date :
June 2013
Event name :
COSM'ING 2013
Event place :
Saint-Malo, France
Event date :
Du 27 juin 2013 au 28 juin 2013
Audience :
International
Name of the research project :
Projet ARC Termitofuel
Available on ORBi :
since 08 August 2013

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