helper T-lymphoyte frequency; bone marrow transplantation; GVHD
Abstract :
[en] Helper T-lymphocyte precursor (HTLp) frequency
from 19 allogeneic bone marrow donors was tested to detect
weak antigenic differences with the recipient, and then
compared to the outcome. HTLp frequency was estimated in
limiting dilution cultures, and HLA-DR and CD 80 expression
by stimulating cells was measured by flow cytometry.
12/19 patients experienced acute graft-versus-host disease
(aGVHD) grade II–IV. A good correlation was found between
high pretransplant HTLp frequency and grade II–IV aGVHD
(median: 1/55848 PBMNC for II–IV GVHD versus 1/
184346 for 0–I GVHD; P¼0·008). Sensitivity was 82%,
specificity 63%, negative predictive value 71% and positive
predictive value 75%. Long-term survivors also had a lower
HTLp median frequency (1/143354) when compared
with patients who died as a result of the transplant
procedure (1/22100, P < 0·001). No correlation was found
between HTLp frequency and HLA-DR or CD80 expression
by patient’s cells. We conclude that HTLp frequency
estimation can predict, although poorly, acute GVHD risk
and long-term survival.
Research center :
Université Catholique de Louvain, Immunohaematology, Brussels
Disciplines :
Hematology
Author, co-author :
Winandy, Marie ; Université Catholique de Louvain - UCL > Faculté de Médecine > Immuno-hématologie
Lewalle, Philippe
Deneys, Véronique
Ferrant, Augustin
De Bruyère, Marc
Language :
English
Title :
Pretransplant helper T-lymphocyte determination in bone marrow donors: acute graft-versus-host disease prediction and relation with long-term survival
Publication date :
1999
Journal title :
British Journal of Haematology
ISSN :
0007-1048
eISSN :
1365-2141
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
105
Pages :
288-294
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
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