Expression of a cDNA clone corresponding to the long open reading frame (XBL-I) of the bovine leukemia virus.

Base Sequence; DNA/genetics; DNA, Recombinant; Leukemia Virus, Bovine/genetics; RNA, Viral/genetics; Recombinant Proteins/biosynthesis/genetics; Retroviridae/genetics; Viral Proteins/biosynthesis/genetics

Abstract :

[en] Nucleotide sequence analysis of a cDNA clone corresponding to the XBL-I open reading-frame of bovine leukemia virus (BLV) revealed that the AUG initiation codon was located 44 bases downstream from that of the env gene and was part of the p34x mRNA splice donor. . .ATGG/GTAA at the end of the pol gene sequence. RNA from this clone was synthesized in vitro by the SP6 RNA polymerase and translated into a 34,000 mol wt protein in rabbit reticulocyte lysates. The protein (p34x) is recognized in Western blots by most sera of BLV-infected sheep and tumor-bearing cattle, by an anti-synthetic peptide rabbit serum, and by the serum of a rabbit immunized by XBL-I RNA programmed reticulocyte lysates. Both sera react with a 34,000 mol wt protein present in nuclei of BLV-infected cells.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Willems, Luc ; Université de Liège - ULiège > Gembloux Agro-Bio Tech

Bruck, C.

Portetelle, Daniel ; Université de Liège - ULiège > Gembloux Agro-Bio Tech

Burny, Arsène ; Université de Liège - ULiège > Agro Biotech Gembloux

Kettmann, Richard ; Université de Liège - ULiège > Gembloux Agro-Bio Tech

Language :

English

Title :

Expression of a cDNA clone corresponding to the long open reading frame (XBL-I) of the bovine leukemia virus.

Publication date :

1987

Journal title :

Virology

ISSN :

0042-6822

eISSN :

1096-0341

Publisher :

Academic Press, San Diego, United States - California

Volume :

160

Issue :

Pages :

55-9

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 26 January 2010

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Bibliography

Aviv, Leder (1972) Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc. Nad. Acad. Sci. USA 69:1408-1412.
Burny, Bruck, Chantrenne, Cleuter, Dekegel, Ghysdael, Kettmann, Leclercc, Leunen, Mammerickx, Portetelle (1980) Bovine leukemia virus: Molecular biology and epidemiology. Viral Oncology , G. Klein, Raven Press, New York; 231-289.
Butler, Chamberlin (1982) Bacteriophage SP6-specific RNA polymerase. J. Biol Chem. 257:5772-5778.
Derse, Caradonna, Casey (1984) Bovine leukemia virus long terminal repeat: A cell-specific promoter. Science 227:317-320.
Derse, Casey (1986) Two elements in the bovine leukemia virus long terminal repeat that regulate gene expression. Science 231:1437-1440.
Deschamps, Kettmann, Burny (1981) Experiments with cloned complete tumor-derived bovine leukemia virus information prove that the virus is totally exogenous to its target animal species. J. Virol. 40:605-609.
Ghysdael, Kettmann, Burny (1979) Translation of bovine leukemia virus virion RNAs in heterologous protein-synthesizing systems. J. Virol. 29:1087-1098.
Glover A Cloning: A Practical Approach, IRL Press, Oxford Washington DC; 1985.
Goh, Sodroski, Rosen, Essex, Haseltine (1985) Subcellular localization of the product of the long open reading frame of human T-cell leukemia virus type I. Science 227:1227-1228.
Graves, Ferrer (1976) In vitro transmission and propagation of the bovine leukemia virus in monolayer cell cultures. Cancer Res. 36:4152-4159.
Green, Brackman, Lucher, Symington, Kramer (1983) Human adenovirus 2 El B-19K and EIB-53K tumor antigens: Antipeptide antibodies targeted to the NH2 and COOH termini. J. Virol. 48:604-615.
Gregoire, Couez, Deschamps, Heuertz, Hors-Cayla, Szpirer, Szpirer, Burny, Huez, Kettmann (1984) Different bovine leukemia virus-induced tumors harbor the provirus in different chromosomes. J. Virol. 50:275-279.
Guo, Wong-Staal, Gallo (1984) Novel viral sequences related to human T-cell leukemia virus in T cells of a seropositive baboon. Science 223:1195.
Haseltine, Sodroski, Patarca, Briggs, Perkins, Wong-Staal (1984) Structure of 3′terminal region of type II human T lymphotropic virus: Evidence for new coding region. Science 225:419-421.
Kettmann, Cleuter, Grégoire, Burny (1985) Role of the 3′ long open reading frame region of bovine leukemia virus in the maintenance of cell transformation. J. Virol. 54:899-901.
Kettmann, Deschamps, Cleuter, Couez, Burny, Marbaix (1982) Leukemogenesis by bovine leukemia virus: Provirus DNA integration and lack of RNA expression of viral long terminal repeat and 3′ proximate cellular sequences. Proc. Natl. Acad. Sci. USA 79:2465-2469.
Kettmann, Deschamps, Couez, Claustriaux, Palm, Burny (1983) Chromosome integration domain for bovine leukemia provirus in tumors. J. Virol. 47:146-150.
Kettmann, Marbaix, Cleuter, Portetelle, Mammerickx, Burny (1980) Genomic integration of bovine leukemia provirus and lack of viral RNA expression in the target cells of cattle with different response to BLV infection. Leuk. Res. 4:509-519.
Mamoun, Astier-Gin, Kettmann, Deschamps, Rebeyrotte, Guillemain (1985) The pX region of the bovine leukemia virus is transcribed as a 2.1-kilobase mRNA. J. Virol. 54:625-629.
Maniatis, Fritsch, Sambrook Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY; 1982.
Melton, Krieg, Rebagliati, Maniatis, Zinn, Green (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic. Acids Res. 12:7035-7056.
Pavlovec, Evenson, Hamilton (1978) The use of guanidine-HCI for the isolation of both RNA and Protein from RNA tumor viruses. J. Gen. Virol. 40:239-243.
Pelham, Jackson (1976) An efficient mRNA-dependent translation system for reticulocyte lysates. Eur. J. Biochem. 67:247-256.
Renart, Sandoval (1984) Western blots. Methods in Enzymology , W.B. Jakoby, Academic Press, Orlando; 104:455-460.
Rice, Stephens, Couez, Deschamps, Kettmann, Burny, Gilden (1984) The nucleotide sequence of the env gene and post-env region of bovine leukemia virus. Virology 138:82-93.
Rosen, Sodroki, Kettmann, Burny, Haseltine (1985) Trans activation of the bovine leukemia virus long terminal repeat in BLV-infected cells. Science 227:320-322.
Rosen, Sodroski, Willems, Kettmann, Campbell, Zaya, Burny, Haseltine (1986) The 3′ region of bovine leukemia virus genome encodes a transactivator protein. Embo J. 5:2585-2589.
Sagata, Yasunaga, Tsuzuku-Kawamura, Ohishi, Ogawa, Ikawa (1985) Complete nucleotide sequence of the genome of bovine leukemia virus: Its evolutionary relationship to other retroviruses. Proc. Natl. Acad. Sci. USA 82:677-681.
Sagata, Tsuzuku-Kawamura, Nagayoshi-Aida, Shimizy, Imagawa, Ikawa (1985) Identification and some biochemical properties of the major XBL gene product of bovine leukemia virus. Proc. Natl. Acad. Sci. USA 82:7879-7883.
Sagata, Yasunaga, Ikawa (1985) Two distinct polypeptides may be translated from a single spliced mRNA of the X genes of human T-cell leukemia and bovine leukemia viruses. FEBS Lett. 192:37-42.
Sanger (1981) Determination of nucleotide sequences in DNA. Science 214:1205-1210.
Seiki, Hattori, Hirayama, Yoshida (1983) Human adult T-cell leukemia virus: A complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA. Proc. Natl. Acad. Sci. USA 80:3618-3622.
Seiki, Hikokoshi Taniguchi, Yoshida (1985) Expression of the px gene of HTLV-I. General splicing mechanism in the HTLV family. Science 228:1532-1534.
Shimotohno, Wachsman, Takahashi, Golde, Miwa, Sugimura, Chen (1984) Nucleotide sequence of the 3′ region of an infectious human T-cell leukemia virus type II genome. Proc. Natl. Acad. Sci. USA 81:6657-6661.
Sodroski, Rosen, Haseltine (1984) Trans-acting transcriptional activation of the long terminal repeat of human T lymphotropic viruses in infected cells. Science 225:381-385.
Wachsman, Golde, Temple, Orr, Clark, Chen (1985) HTLV-x-gene product: Requirement for the env methionine initiation codon. Science 228:1534-1537.
Yoshinaka, Oroszlan (1985) Bovine leukemia virus post-envelope gene coded protein: Evidence for expression in natural infection. Biochem. Biophys. Res. Commun. 131:347-354.