Reference : Expression of a cDNA clone corresponding to the long open reading frame (XBL-I) of th...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/15235
Expression of a cDNA clone corresponding to the long open reading frame (XBL-I) of the bovine leukemia virus.
English
Willems, Luc mailto [Université de Liège - ULg > > Gembloux Agro-Bio Tech >]
Bruck, C. [> > > >]
Portetelle, Daniel mailto [Université de Liège - ULg > > Gembloux Agro-Bio Tech >]
Burny, A. [> > > >]
Kettmann, Richard mailto [Université de Liège - ULg > > Gembloux Agro-Bio Tech >]
1987
Virology
Academic Press
160
1
55-9
Yes (verified by ORBi)
International
0042-6822
1096-0341
San Diego
CA
[en] Base Sequence ; DNA/genetics ; DNA, Recombinant ; Leukemia Virus, Bovine/genetics ; RNA, Viral/genetics ; Recombinant Proteins/biosynthesis/genetics ; Retroviridae/genetics ; Viral Proteins/biosynthesis/genetics
[en] Nucleotide sequence analysis of a cDNA clone corresponding to the XBL-I open reading-frame of bovine leukemia virus (BLV) revealed that the AUG initiation codon was located 44 bases downstream from that of the env gene and was part of the p34x mRNA splice donor. . .ATGG/GTAA at the end of the pol gene sequence. RNA from this clone was synthesized in vitro by the SP6 RNA polymerase and translated into a 34,000 mol wt protein in rabbit reticulocyte lysates. The protein (p34x) is recognized in Western blots by most sera of BLV-infected sheep and tumor-bearing cattle, by an anti-synthetic peptide rabbit serum, and by the serum of a rabbit immunized by XBL-I RNA programmed reticulocyte lysates. Both sera react with a 34,000 mol wt protein present in nuclei of BLV-infected cells.
http://hdl.handle.net/2268/15235
also: http://hdl.handle.net/2268/25436

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