Article (Scientific journals)
Insights into bacterial cellulose biosynthesis by functional metagenomics on Antarctic soil samples.
Berlemont, Renaud; Delsaute, Maud; Pipers, Delphine et al.
2009In ISME Journal, 3 (9), p. 1070-1081
Peer Reviewed verified by ORBi
 

Files


Full Text
ISMEJ_2009_Berlemont.pdf
Publisher postprint (358.47 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] In this study, the mining of an Antarctic soil sample by functional metagenomics allowed the isolation of a cold-adapted protein (RBcel1) that hydrolyzes only carboxymethyl cellulose. The new enzyme is related to family 5 of the glycosyl hydrolase (GH5) protein from Pseudomonas stutzeri (Pst_2494) and does not possess a carbohydrate-binding domain. The protein was produced and purified to homogeneity. RBcel1 displayed an endoglucanase activity, producing cellobiose and cellotriose, using carboxymethyl cellulose as a substrate. Moreover, the study of pH and the thermal dependence of the hydrolytic activity shows that RBcel1 was active from pH 6 to pH 9 and remained significantly active when temperature decreased (18% of activity at 10 degrees C). It is interesting that RBcel1 was able to synthetize non-reticulated cellulose using cellobiose as a substrate. Moreover, by a combination of bioinformatics and enzyme analysis, the physiological relevance of the RBcel1 protein and its mesophilic homologous Pst_2494 protein from P. stutzeri, A1501, was established as the key enzymes involved in the production of cellulose by bacteria. In addition, RBcel1 and Pst_2494 are the two primary enzymes belonging to the GH5 family involved in this process.The ISME Journal advance online publication, 21 May 2009; doi:10.1038/ismej.2009.48.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Berlemont, Renaud ;  Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Delsaute, Maud 
Pipers, Delphine ;  Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
D'Amico, Salvino ;  Université de Liège - ULiège > GIGA-Research
Feller, Georges ;  Université de Liège - ULiège > Département des sciences de la vie > Labo de biochimie
Galleni, Moreno ;  Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Power, Pablo ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Language :
English
Title :
Insights into bacterial cellulose biosynthesis by functional metagenomics on Antarctic soil samples.
Publication date :
2009
Journal title :
ISME Journal
ISSN :
1751-7362
eISSN :
1751-7370
Publisher :
Nature Publishing Group, United Kingdom
Volume :
3
Issue :
9
Pages :
1070-1081
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 26 January 2010

Statistics


Number of views
245 (42 by ULiège)
Number of downloads
322 (12 by ULiège)

Scopus citations®
 
47
Scopus citations®
without self-citations
36
OpenCitations
 
40

Bibliography


Similar publications



Contact ORBi