In vivo administration of acepromazine or promethazine to horse decreases the reactive oxygen species production response of subsequently isolated neutrophils to stimulation with phorbol myristate acetate
[en] The previous experiments have shown that some phenothiazines have
antioxidant and anti-inflammatory properties in vitro. In this study the
inhibition of the production of reactive oxygen species (ROS) by neutrophils
was studied in two groups of horses, which received a dose of 0.1 mg⁄ kg of
either acepromazine or promethazine intravenously. Blood samples were
collected before (T0) and 0.5, 1, 3 and 5 h after drug administration. The
chemiluminescence (CML) response of neutrophils was measured ex vivo in the
presence of luminol for a period of 10 min and the maximum CML value (peak
value) recorded. There was a significant inhibition of the ROS production in the
acepromazine treated group (49% inhibition) at 5 h after administration and in
the promethazine group (24% inhibition) at 3 h after administration (P < 0.05
vs. T0). These findings are of therapeutic relevance in the use of phenothiazines
in equine patients with inflammatory diseases where neutrophil activation and
ROS production are implicated.
Research center :
CORD - Centre de l'Oxygène, Recherche et Développement - ULiège
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Péters, Fabrice
Franck, Thierry ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Pequito, Manuel
de la Rebière de Pouyade, Geoffroy ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Grulke, Sigrid ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés
Salciccia, Alexandra ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés
Verwilghen, Denis ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Chiavaccini, Ludovica
Deby, Ginette ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Serteyn, Didier ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
In vivo administration of acepromazine or promethazine to horse decreases the reactive oxygen species production response of subsequently isolated neutrophils to stimulation with phorbol myristate acetate
Publication date :
2009
Journal title :
Journal of Veterinary Pharmacology and Therapeutics
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