Poster (Scientific congresses and symposiums)
User-friendly method for GCxGC optimization
Stefanuto, Pierre-Hugues; Dimandja, Jean-Marie; Focant, Jean-François
2013International Symposium of Capillary Chromatography
 

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Keywords :
GCxGC; Optimization; Orthogonality Index
Abstract :
[en] Almost 30 years are gone since the first paper about multidimensional GC was published by John B. Phillips 1. After several years of developing process, Comprehensive Two Dimensional Gas Chromatography systems are in the commercialization step of its live time. During this period, the advantages of GC×GC, regarding to classical D1 system, were clearly demonstrated 2. However, the new parameters involve in a GC×GC method are still not completely understand and people don’t use it at the maximum of its capacity. According to the literature more and more group are using GC×GC for different kind of applications. In most of these researches, GC×GC was used for its separation power. Unfortunately, the orthogonality and the column set are most of the time poorly optimized. To help people in this important step, we develop an user-friendly method to choose the best column combination and the best separation parameters for a particular application. All these developments were based on different mix of standard call the Century mix and the Dimandja mix. These are the descendants of the Phillips mix create in 2003 by J. Dimandja 3. These mixtures contain homologous series of compounds representing the polarity and the volatility range commonly sees in GC×GC. This method is based on the Retention Index obtained in a classical GC analysis and projected in the 2D space. Using this projection method, we identified four major types of orthogonality based on the peak dispersion obtained. We name those: Normal, Reverse, Hybrid and Transpose orthogonality. To characterize these observations, we developed the Orthogonality Index. Going back to the mathematical definition of orthogonality, this factor is the angle formed between the alkane line and the aromatic hydrocarbon line in the chromatographic space. Using this predictive tool, people should be able to choose the best column set and to optimize easily the separation parameters. 1. Phillips, J.B., Luu, D., Pawliszyn, J.B. & Carle, G.C. Multiplex gas chromatography by thermal modulation of a fused silica capillary column. Anal. Chem. 57, 2779–2787 (1985). 2. Dimandja, J.-M.D. Comprehensive 2-D GC provides high-performance separations in terms of selectivity, sensitivity, speed, and structure. Anal. Chem. 76, 167A–174A (2004). 3. Dimandja, J., Clouden, G. & Colón, I. Standardized test mixture for the characterization of comprehensive two-dimensional gas chromatography columns: the Phillips mix. J Chromatogr a 1019, 261–272 (2003).
Research center :
Organic and Biological Analytical Chemistry Group
Disciplines :
Chemistry
Author, co-author :
Stefanuto, Pierre-Hugues  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique, organique et biologique
Dimandja, Jean-Marie
Focant, Jean-François  ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique, organique et biologique
Language :
English
Title :
User-friendly method for GCxGC optimization
Publication date :
May 2013
Event name :
International Symposium of Capillary Chromatography
Event organizer :
CASSS
Event place :
Palm Springs, United States
Event date :
du 12 mai 2013 au 16 mai 2013
Audience :
International
Funders :
ULiège - Université de Liège [BE]
Available on ORBi :
since 24 June 2013

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